The problem of a growing resistance of bacteria and other microorganisms to conventional antibiotics gave rise to a search for new potent antimicrobial agents. Insect antimicrobial peptides (AMPs) seem to be promising novel potential anti-infective therapeutics. The dipeptide β-alanyl-tyrosine (β-Ala-Tyr) is one of the endogenous insect toxins exhibiting antibacterial activity against both Gram-negative and Gram-positive bacteria. Prior to testing its other antimicrobial activities, it has to be prepared in a pure form. In this study, we have developed a capillary zone electrophoresis (CZE) method for analysis of β‑Ala‑Tyr isolated from the extract of the hemolymph of larvae of the fleshfly Neobellieria bullata by reversed-phase high-performance liquid chromatography (RP-HPLC). Based on our previously described correlation between CZE and free-flow zone electrophoresis (FFZE), analytical CZE separation of β‑Ala‑Tyr and its admixtures have been converted into preparative purification of β‑Ala‑Tyr by FFZE with preparative capacity of 45.5 mg per hour. The high purity degree of the β‑Ala‑Tyr obtained by FFZE fractionation was confirmed by its subsequent CZE analysis.

中文翻译：

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毛细管和自由流动区带电泳在新贝氏肉蝇血淋巴中抗菌素β-丙氨酰酪氨酸的分析和纯化中的应用

细菌和其他微生物对常规抗生素的耐药性不断增加的问题引起了对新的强效抗菌剂的研究。昆虫抗微生物肽 (AMP) 似乎是有前途的新型潜在抗感染疗法。二肽 β-丙氨酰酪氨酸 (β-Ala-Tyr) 是一种内源性昆虫毒素，对革兰氏阴性菌和革兰氏阳性菌均具有抗菌活性。在测试其其他抗菌活性之前，必须以纯形式制备。在本研究中，我们开发了一种毛细管区带电泳 (CZE) 方法，用于分析从肉蝇 Neobellieriabulata幼虫血淋巴提取物中分离的 β-Ala-Tyr通过反相高效液相色谱 (RP-HPLC)。基于我们之前描述的 CZE 和自由流动区带电泳 (FFZE) 之间的相关性，β-Ala-Tyr 及其混合物的分析性 CZE 分离已被 FFZE 转化为制备能力为 45.5 mg 的 β-Ala-Tyr 的制备纯化每小时。随后的 CZE 分析证实了通过 FFZE 分馏获得的 β-Ala-Tyr 的高纯度。