Diosmetin is a citrus flavonoid that has antioxidant and anti-inflammatory effects. This study examined the effect of diosmetin on blood pressure and vascular alterations and its underlying mechanisms in experimentally hypertensive rats. Male Sprague rats were administered Nω-nitro-L-arginine methyl ester L-NAME for five weeks and were given diosmetin at doses of 20 or 40 mg/kg or captopril (5 mg/kg) for two weeks. Diosmetin alleviated hypertension, improved endothelial dysfunction, and suppressed the overactivity of sympathetic nerve-mediated vasoconstriction in aorta and mesentery hypertensive rats (p < 0.05). Increases in plasma and aortic tissue malondialdehyde (MDA) and carotid superoxide generations and reductions of plasma superoxide dismutase, catalase, and nitric oxide in hypertensive rats were ameliorated by diosmetin (p < 0.05). Diosmetin increased the protein expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) in hypertensive rats. Furthermore, diosmetin mitigated hypertrophy and collagen accumulation of the aortic wall in L-NAME rats. It exhibited an anti-inflammatory effect by reducing interleukin-6 (IL-6) accumulation and by overexpressing the phospho-c-Jun N-terminal kinases (p-JNK) and the phospho-nuclear factor-kappaB (p-NF-κB) proteins in the aorta (p < 0.05). Captopril was a positive control substance and had similar effects to diosmetin. In summary, diosmetin reduced blood pressure and alleviated vascular abnormalities in L-NAME-treated rats. These effects might be related to antioxidant and anti-inflammatory effects as well as to the modulation of the expression of the Nrf2/HO1 and p-JNK/NF-κB proteins.

中文翻译：

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Diosmetin 通过调节高血压大鼠的 Nrf2/HO-1 和 p-JNK/p-NF-κB 表达改善血管功能障碍和重塑

Diosmetin 是一种柑橘类黄酮，具有抗氧化和抗炎作用。本研究检查了地奥司美汀对实验性高血压大鼠的血压和血管改变的影响及其潜在机制。雄性 Sprague 大鼠被给予 Nω-硝基-L-精氨酸甲酯 L-NAME 5 周，并以 20 或 40 mg/kg 或卡托普利 (5 mg/kg) 的剂量给予地奥美汀两周。地奥司他汀减轻主动脉和肠系膜高血压大鼠的高血压，改善内皮功能障碍，抑制交感神经介导的血管收缩的过度活动（p< 0.05)。地奥美汀可改善高血压大鼠血浆和主动脉组织丙二醛 (MDA) 和颈动脉超氧化物生成的增加以及血浆超氧化物歧化酶、过氧化氢酶和一氧化氮的减少 ( p < 0.05)。Diosmetin 增加了高血压大鼠核因子红细胞 2 相关因子 2 (Nrf2) 和血红素加氧酶-1 (HO-1) 的蛋白质表达。此外，地奥美汀减轻了 L-NAME 大鼠主动脉壁的肥大和胶原蛋白积累。它通过减少白细胞介素 6 (IL-6) 积累和过表达磷酸-c-Jun N-末端激酶 (p-JNK) 和磷酸核因子-κB (p-NF-κB) 表现出抗炎作用) 主动脉中的蛋白质 ( p< 0.05)。卡托普利是阳性对照物质，其作用与地奥司美丁相似。总之，地奥美汀降低了 L-NAME 治疗大鼠的血压并减轻了血管异常。这些作用可能与抗氧化和抗炎作用以及调节 Nrf2/HO1 和 p-JNK/NF-κB 蛋白的表达有关。