Pulsed electric fields (PEFs) had the potential to be a new pasteurization technology due to its advantages of energy-saving, high efficiency, and less influence on food quality based on the electroporation effect of PEF on microorganisms. At the same time, temperature played a significant role in the electroporation process. We established a two-membrane dielectric model that coupled temperature and PEF to study the influence of temperature on the dynamic process of electroporation. In this model, we considered both the effect of Joule heat on temperature generated by PEF and the effect of temperature on the conductivity of suspension, cytoplasm, nucleus, the characteristic voltage of electroporation, and molecules transport. The simulation results showed that under the higher ambient temperature, the transmembrane voltage of the cell membrane after electroporation could be stabilized at a higher value, the maximum value of membrane conductivity and pore density on the cell membrane was higher. The relationship between pore radius and temperature was simulated. What is more, temperature facilitated molecules transport, which intensified the destruction of cell homeostasis.

中文翻译：

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基于有限元分析的温度对电穿孔动态过程影响的模拟

基于脉冲电场对微生物的电穿孔作用，脉冲电场（PEFs）具有节能、高效、对食品质量影响小等优点，有望成为一种新型巴氏杀菌技术。同时，温度在电穿孔过程中起到了重要作用。我们建立了耦合温度和PEF的两膜介电模型来研究温度对电穿孔动态过程的影响。在这个模型中，我们考虑了焦耳热对 PEF 产生的温度的影响，以及温度对悬浮液、细胞质、细胞核、电穿孔特征电压和分子传输电导率的影响。仿真结果表明，在较高的环境温度下，电穿孔后细胞膜的跨膜电压可以稳定在较高的值，细胞膜上的膜电导率和孔密度的最大值较高。模拟了孔隙半径与温度之间的关系。更重要的是，温度促进了分子运输，从而加剧了细胞稳态的破坏。