This work reports the enhancement of a single-mode tapered optical fiber (TOF) sensor for Dengue virus (DENV) II E protein using chitosan. Chitosan, an organic nanomaterial with excellent biocompatibility and abundant binding sites, was deposited on TOF as an intermediate layer between the activated taper surface and the anti-DENV II E protein antibodies. The best DENV sensing performance was attained at chitosan thickness of 17.13 nm, which was attributed to the optimum balance between the auxiliary layer thickness and penetration depth of the evanescent field. An enhanced sensitivity value of 14.19 nm/nM was achieved compared to the sensitivity obtained by standard configuration sensor. A lower dissociation constant, ${\text{K}_{d}}$ of $1.00\times 10^{-10}$ M was also acquired, which implies a higher affinity towards the targeted E protein. Findings from this work highlight the potential of organic nanomaterial such as chitosan for biosensor enhancement and further emphasize the capability of fiber optic sensors for detection of dengue virus as well as other antigens.

中文翻译：

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壳聚糖集成单模锥形光纤 DENV II E 蛋白质传感器

这项工作报告了使用壳聚糖对登革热病毒 (DENV) II E 蛋白的单模锥形光纤 (TOF) 传感器的增强。壳聚糖是一种具有优异生物相容性和丰富结合位点的有机纳米材料，沉积在 TOF 上，作为活化的锥形表面和抗 DENV II E 蛋白抗体之间的中间层。在 17.13 nm 的壳聚糖厚度下获得最佳 DENV 传感性能，这归因于辅助层厚度和渐逝场穿透深度之间的最佳平衡。与标准配置传感器获得的灵敏度相比，灵敏度提高了 14.19 nm/nM。较低的解离常数， ${\text{K}_{d}}$ 的 $1.00\乘以 10^{-10}$ 还获得了 M，这意味着对目标 E 蛋白的亲和力更高。这项工作的发现突出了有机纳米材料（如壳聚糖）在生物传感器增强方面的潜力，并进一步强调了光纤传感器检测登革热病毒和其他抗原的能力。