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Anthelmintic potency of Rumex crispus L. extracts against Caenorhabditis elegans and non-targeted identification of the bioactive compounds
Saudi Journal of Biological Sciences ( IF 4.4 ) Pub Date : 2021-09-17 , DOI: 10.1016/j.sjbs.2021.09.026
Oladayo Amed Idris 1, 2 , Olubunmi Abosede Wintola 1 , Anthony Jide Afolayan 1
Affiliation  

Traditional healers and ethnoveterinary therapists use several medicinal plants, such as Rumex crispus L., to treat endoparasite infections. R. crispus has been established by researchers to be effective agasint a few parasitic worms. In this study, we evaluated the potency of R. crispus extracts on the model organism, Caenorhabditis elegans and the bioactive compounds of the extracts were also identified. The solvent extracts of R. crispus were tested against C. elegans for up to 72 h. The effect of the extracts on C. elegans was examined using light microscopy (LM) and scanning electron microscopy (SEM). LM and SEM analysis showed damage on the body wall, reduced body and slight modifications of the nematode organs. The lethality test reveals a significant reduction in the viability of the nematode with the water extract of leaf (LF-WAE), among others, having the strongest potency against the nematode, with 83% lethality. Anlysis done with Fourier-transform infrared spectroscopy (FTIR) spectra reveals various characteristic vibration bands and fingerprint bands at 3400–600 cm−1, identifying phenols, organic acids, aromatics, amines, among others in the plant. The compounds were identified with liquid chromatography-mass spectrometry (LC-MS), under the categories of flavonoids, steroidal alkaloids and proanthocyanidin. In conclusion, this study confirmed that R. crispus has anthelmintic potential, using standardised C. elegans models as a tool and suggests that there could be novel compounds yet to be explored in the studied plant that could be of great benefit to livestock and humans.



中文翻译:

Rumex crispus L. 提取物对秀丽隐杆线虫的驱虫效力和生物活性化合物的非靶向鉴定

传统治疗师和民族兽医治疗师使用几种药用植物,如Rumex crispus L.,来治疗体内寄生虫感染。研究人员已经确定R. crispus可以有效对抗一些寄生虫。在这项研究中,我们评估了R.crispus提取物对模式生物秀丽隐杆线虫的效力,并且还鉴定了提取物的生物活性化合物。R.crispus的溶剂提取物针对C. elegans进行了长达 72 小时的测试。提取物对秀丽隐杆线虫的影响使用光学显微镜(LM)和扫描电子显微镜(SEM)检查。LM 和 SEM 分析显示体壁受损,体减少,线虫器官略有改变。致死率测试表明,叶水提取物 (LF-WAE) 可显着降低线虫的活力,其中对线虫具有最强的效力,致死率为 83%。用傅里叶变换红外光谱 (FTIR) 光谱进行的分析揭示了 3400–600 cm -1处的各种特征振动带和指纹带,识别植物中的酚类、有机酸、芳烃、胺等。在黄酮类、甾体生物碱和原花青素类别下,用液相色谱-质谱 (LC-MS) 鉴定了这些化合物。总之,这项研究使用标准化的秀丽隐杆线虫模型作为工具,证实了R.crispus具有驱虫潜力,并表明在所研究的植物中可能存在有待探索的新化合物,这些化合物可能对牲畜和人类大有裨益。

更新日期:2021-09-17
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