In the present study, two Epichloë sinensis endophyte strains isolated from different Festuca sinensis ecotypes were inoculated on potato dextrose agar (PDA) and potato dextrose broth (PDB) media with or without (control) exogenous additives. After 4weeks of growth, the growth (colony diameter, hyphal diameter, and mycelial biomass) and other characteristics (pH and antioxidant capacity of culture filtrate, mycelial ion contents, and hormone contents) were measured. The results showed that the culture conditions had significant effects (p<0.05) on the hyphal diameter, mycelial biomass, and hormone content of the two strains. The mycelial biomass of the two strains in PDB was significantly higher (p<0.05) than that on PDA. Except for strain 1 with indole-3-acetic acid (IAA) treatment and strain 84F with control and VB₁ treatments, the hyphal diameter of the two strains in PDB under the other treatments was significantly higher (p<0.05) than that on PDA. In most cases, the IAA, cytokinins (CTK), abscisic acid (ABA), and gibberlic acid (GA) contents in the mycelia on PDA of the two strains were significantly higher (p<0.05) than those in PDB. The two E. sinensis strains exhibited significantly different performances (p<0.05) under the five treatments. The indices, including colony diameter, mycelial biomass, scavenging ability of superoxide anion radicals and hydroxyl radicals, pH of culture filtrate, ion contents, hyphal diameter, and IAA, CTK, GA, and ABA contents were significantly different (p<0.05) between the two strains, although the performance was inconsistent. Exogenous additives had significant effects (p<0.05) on the performance of the two E. sinensis strains. Indole-3-acetic acid and VB₁ treatments significantly promoted (p<0.05) the growth of the two strains on both PDA and PDB. Indole-3-acetic acid treatment also significantly increased the hyphal diameters of the two strains in PDB (p<0.05). Indole-3-acetic acid and VB₁ treatments significantly reduced (p<0.05) the antioxidant ability of these two strains in PDB. NaCl and ZnCl₂ treatments had significant inhibitory effects (p<0.05) on fungal growth and promotion effects on the antioxidant ability of the two strains. The treatments also had significant effects (p<0.05) on hyphal diameters and ion and hormone contents, although the effects varied with different indices.

在本研究中，两个华灯笼草分离自不同种属的内生菌菌株羊茅将生态型接种在有或没有（对照）外源添加剂的马铃薯葡萄糖琼脂（PDA）和马铃薯葡萄糖肉汤（PDB）培养基上。生长4周后，测量生长（菌落直径、菌丝直径和菌丝生物量）和其他特性（培养滤液的pH和抗氧化能力、菌丝离子含量和激素含量）。结果表明，培养条件有显着影响（p<0.05）对两个菌株的菌丝直径、菌丝生物量和激素含量的影响。PDB中两个菌株的菌丝生物量显着更高（p<0.05) 与 PDA 上的结果相比。除吲哚-3-乙酸（IAA）处理的菌株1和对照和VB ₁处理的菌株84F外，其他处理下PDB中的两个菌株的菌丝直径均显着较高（p<0.05) 与 PDA 上的结果相比。大多数情况下，两个菌株PDA上菌丝体中的IAA、细胞分裂素（CTK）、脱落酸（ABA）和赤霉酸（GA）含量均显着较高（p<0.05) 与 PDB 中的相比。他们俩中华桉菌株表现出显着不同的性能（p<0.05）在五个处理下。菌落直径、菌丝生物量、超氧阴离子自由基和羟自由基清除能力、培养滤液pH、离子含量、菌丝直径以及IAA、CTK、GA、ABA含量等指标差异显着（p<0.05），尽管性能不一致，但两个菌株之间存在差异。外源添加剂有显着影响（p<0.05) 两者的性能中华桉菌株。Indole-3-乙酸和 VB ₁处理显着促进（p<0.05）两种菌株在PDA和PDB上的生长。吲哚-3-乙酸处理还显着增加了 PDB 中两个菌株的菌丝直径（p<0.05）。Indole-3-乙酸和 VB ₁处理显着降低（p<0.05）这两个菌株在PDB中的抗氧化能力。NaCl和ZnCl ₂处理具有显着的抑制作用（p<0.05)对两种菌株的真菌生长和抗氧化能力的促进作用。这些治疗也产生了显着的效果（p<0.05）对菌丝直径以及离子和激素含量的影响，尽管不同指标的影响有所不同。