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Effect of the Histone Methyltransferase Specific Probe BRD4770 on Metabolic Profiling of the Endophytic Fungus Diaporthe longicolla
Frontiers in Microbiology ( IF 5.2 ) Pub Date : 2021-09-17 , DOI: 10.3389/fmicb.2021.725463
Jay Hind Nishad 1 , Arti Singh 1 , Rajnish Bharti 1 , Priyanka Prajapati 1 , Vijay Kumar Sharma 2 , Vijai Kumar Gupta 3 , Ravindra Nath Kharwar 1
Affiliation  

The endophytic fungus Diaporthe longicolla was isolated from the stem of Saraca asoca (Roxb.) Willd., commonly known as Ashok plant in India and Sri Lanka. Since no reports are available regarding epigenetic modulations by BRD4770 in microbial entities, D. longicolla was treated with different concentrations of BRD4770 for this purpose and evaluated for its antioxidant and antibacterial potential against five human pathogenic bacteria, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Shigella boydii, Klebsiella pneumoniae, and Escherichia coli. The crude extract obtained from cultures treated with 100 nM concentration of BRD4770 showed increased antioxidant activity and inhibition zone against S. aureus and MRSA, compared to the non-treated control. The composition of the non-treated and treated crude extract was analyzed, and induced compounds were identified with the help of Gas chromatography–mass spectrometry (GC-MS) and LC-ESI-MS/MS. LC-ESI-MS/MS analysis showed that berberine (antibacterial)-, caffeine-, and theobromine (antioxidant)-like compounds were induced in the BRD4770-treated crude extract. The presence of particular absorbance at a wavelength of 346.5 nm for berberine, 259.4 nm for caffeine, and 278.4 nm for theobromine in the reverse-phase high-performance liquid chromatography (HPLC) analysis of both BRD4770-treated crude metabolites and standard solution of the above compounds strongly supported the increased antibacterial and antioxidant activities that may be due to inducing the alterations in bioactivities of the BRD4770-treated culture.



中文翻译:

组蛋白甲基转移酶特异性探针 BRD4770 对内生真菌 Diaporthe longicolla 代谢谱的影响

内生真菌长叶甘草从茎中分离出来萨拉卡·阿索卡(Roxb.) Willd.,在印度和斯里兰卡俗称 Ashok 植物。由于没有关于 BRD4770 对微生物实体的表观遗传调节的报告,D长根科拉为此用不同浓度的 BRD4770 进行处理,并评估其针对五种人类致病菌的抗氧化和抗菌潜力,金黄色葡萄球菌, 耐甲氧西林金黄色葡萄球菌(耐甲氧西林金黄色葡萄球菌),博伊迪志贺菌、肺炎克雷伯菌, 和大肠杆菌。从用 100 nM 浓度的 BRD4770 处理的培养物中获得的粗提物显示出增加的抗氧化活性和抑制区域金黄色葡萄球菌和 MRSA,与未处理的对照相比。对未经处理和处理的粗提物的成分进行了分析,并借助气相色谱-质谱法 (GC-MS) 和 LC-ESI-MS/MS 鉴定了诱导化合物。LC-ESI-MS/MS 分析表明,经过 BRD4770 处理的粗提物中诱导出了小檗碱(抗菌)、咖啡因和可可碱(抗氧化剂)类化合物。在对 BRD4770 处理的粗代谢物和标准溶液进行反相高效液相色谱 (HPLC) 分析时,小檗碱在 346.5 nm 波长、咖啡因在 259.4 nm 波长、可可碱在 278.4 nm 波长处存在特定吸光度上述化合物强烈支持抗菌和抗氧化活性的增加,这可能是由于诱导 BRD4770 处理的培养物生物活性的改变所致。

更新日期:2021-09-17
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