Our previous study proved that Salvia miltiorrhiza could enhance fat graft survival by promoting adipogenesis. However, the effect of salvianolic acid B (Sal-B), the most abundant and bioactive water-soluble compound in Salvia miltiorrhiza, on fat graft survival has not yet been investigated. This study aims to investigate whether salvianolic acid B could improve fat graft survival and promote preadipocyte differentiation. The underlying mechanism has also been studied. In vivo, 0.2 ml of Coleman fat was transplanted into nude mice with salvianolic acid B. The grafts were evaluated by HE and IF at 2 and 4 weeks posttransplantation and by micro-CT at 4 weeks posttransplantation. In vitro, the adipogenesis and proliferative activities of salvianolic acid B were analyzed in cultured human adipose-derived stem cells (h-ADSCs) and 3T3-L1 cells to detect the mechanism by which salvianolic acid B affects graft survival. In vivo, the weights and volumes of the fat grafts in the Sal-B-treated groups were significantly higher than those of the fat grafts in the control group. In addition, higher fat integrity and more viable adipocytes were observed in the Sal-B-treated groups. In vitro, salvianolic acid B showed the ability to promote 3T3-L1 and h-ADSC proliferation and adipogenesis. Our in vitro experiments demonstrated that salvianolic acid B can promote the proliferation of adipose stem cells and enhance the differentiation of adipose stem cells. Simultaneously, in vivo experiments showed that salvianolic acid B can improve the survival rate of fat transplantation. Therefore, our research shed light on the potential therapeutic usage of salvianolic acid B in improving the survival rate of fat transplantation.

中文翻译：

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Salvianolic acid-B 通过促进增殖和脂肪生成来提高脂肪移植物的存活率

我们之前的研究证明，丹参可以通过促进脂肪生成来提高脂肪移植物的存活率。然而，丹酚酸 B (Sal-B) 是丹参中含量最丰富且具有生物活性的水溶性化合物，它对脂肪移植物存活的影响尚未研究。本研究旨在调查丹酚酸 B 是否可以提高脂肪移植物的存活率并促进前脂肪细胞分化。还研究了潜在的机制。在体内，将 0.2 ml Coleman 脂肪与丹酚酸 B 一起移植到裸鼠体内。移植后 2 和 4 周通过 HE 和 IF 评估移植物，移植后 4 周通过显微 CT 评估移植物。体外，在培养的人脂肪干细胞 (h-ADSCs) 和 3T3-L1 细胞中分析丹酚酸 B 的脂肪生成和增殖活性，以检测丹酚酸 B 影响移植物存活的机制。在体内，Sal-B 处理组的脂肪移植物的重量和体积显着高于对照组的脂肪移植物。此外，在 Sal-B 治疗组中观察到更高的脂肪完整性和更有活力的脂肪细胞。在体外，丹酚酸 B 显示出促进 3T3-L1 和 h-ADSC 增殖和脂肪生成的能力。我们的体外实验表明，丹酚酸B可以促进脂肪干细胞的增殖，增强脂肪干细胞的分化。同时，体内实验表明丹酚酸B可以提高脂肪移植的成活率。因此，我们的研究揭示了丹酚酸 B 在提高脂肪移植存活率方面的潜在治疗用途。