This work addresses the testing of two newly produced biomass carriers (micro- and nanofibers) and one commercially available AnoxKaldnes™ K3 carrier in a laboratory post-nitrification reactor. The carriers were prepared under parameters suitable for high-quality biomass adhesion to their surface, and each was characterized by its specific structures. As part of the evaluation of the biofilms using respirometry and molecular genetic methods, the carriers were assessed in terms of their effectiveness and comparability. The rate of biofilm development was dependent on the structure and surface properties of the individual carriers. The results showed that the biofilm most strongly adhered to nanofiber carriers, where nitrating bacteria's slower but more abundant development occurred. Microfiber carriers were more stable, but a diverse internal structure may be unsuitable in a populated carrier's early stages. The AnoxKaldnes™ K3 carriers showed the slowest growth of biofilm, but the monitored nitrifying bacteria were abundant after an extended time. AOB representatives are likely to prefer an environment with a high amount of biomass and a large active area. Conversely, NOB representatives thrive better in a slowly forming biofilm. The methods used to monitor biofilm are challenging to compare directly, but they do complement each other, which aids in verifying the individual test results. Developing new types of biomass carriers with the potential for high-quality adhesion of microorganisms is a prerequisite for the expansion of highly efficient biotechnological processes, especially for wastewater treatment.

这项工作涉及在实验室后硝化反应器中测试两种新生产的生物质载体（微米和纳米纤维）和一种市售 AnoxKaldnes™ K3 载体。载体是在适合高​​质量生物质粘附到其表面的参数下制备的，每个载体都以其特定的结构为特征。作为使用呼吸测量法和分子遗传学方法评估生物膜的一部分，对载体的有效性和可比性进行了评估。生物膜发展的速度取决于单个载体的结构和表面特性。结果表明，生物膜最牢固地粘附在纳米纤维载体上，其中硝化细菌的生长速度较慢，但​​发育更丰富。超细纤维载体更稳定，但多样化的内部结构可能不适合在人口密集的航母的早期阶段。AnoxKaldnes™ K3 载体表现出生物膜的最慢增长，但经过长时间监测后，硝化细菌丰富。AOB 代表可能更喜欢具有大量生物量和大活动区域的环境。相反，NOB 代表在缓慢形成的生物膜中茁壮成长。用于监测生物膜的方法很难直接进行比较，但它们确实相互补充，这有助于验证单个测试结果。开发具有微生物高质量粘附潜力的新型生物质载体是扩展高效生物技术过程的先决条件，尤其是废水处理。