Dementia is exacerbated by loss of appetite and amotivation, and recent studies have indicated that ninjin'yoeito improves anorexia and amotivation. Previous studies suggest that ninjin'yoeito inhibits dopamine-metabolizing enzymes and enhances dopamine signaling. However, whether ninjin'yoeito increases dopamine content in living cells remains unclear. Here, PC12 cells were used to examine whether ninjin'yoeito affects the dopamine metabolic pathway. Dopamine content significantly increased 3 h after treatment ninjin'yoeito extract. Concomitantly, the levels of 3-methoxytyramine and 3,4-dihydroxyphenylacetic acid were significantly reduced. The effects of components of ninjin'yoeito on the dopamine metabolic pathway were also assessed. Treatment with onjisaponin B, nobiletin, and schisandrin, and the ingredients of Polygalae Radix, Citri Unshiu Pericarpium, and Schisandrae Fructus increased dopamine content and decreased its metabolite content in the culture media. Our findings suggest that ninjin'yoeito improves anorexia and amotivation by inhibiting metabolic enzyme and increasing the dopamine content in cells.

中文翻译：

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Ninjin'yoeito 是一种日本传统药物，可增加 PC12 细胞中的多巴胺含量。

食欲不振和缺乏动力会加剧痴呆症，最近的研究表明，ninjin'yoeito 可以改善厌食和缺乏动力。先前的研究表明，ninjin'yoeito 抑制多巴胺代谢酶并增强多巴胺信号传导。然而，ninjin'yoeito 是否会增加活细胞中的多巴胺含量仍不清楚。在这里，PC12 细胞用于检查 ninjin'yoeito 是否影响多巴胺代谢途径。忍金洋楸提取物处理后3小时多巴胺含量显着增加。同时，3-甲氧基酪胺和3,4-二羟基苯乙酸的水平显着降低。还评估了 ninjin'yoeito 成分对多巴胺代谢途径的影响。用 onjisaponin B、nobiletin 和五味子素治疗，远志、陈皮、五味子的成分增加了培养基中多巴胺的含量，降低了其代谢产物的含量。我们的研究结果表明，ninjin'yoeito 通过抑制代谢酶和增加细胞中的多巴胺含量来改善厌食和动力不足。