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Endophytic fungi of salt-tolerant plants: diversity and ability to promote plant growth.
Journal of Microbiology and Biotechnology ( IF 2.8 ) Pub Date : 2021-09-15 , DOI: 10.4014/jmb.2106.06007
Irina Khalmuratova 1 , Doo-Ho Choi 1 , Jong-Guk Kim 1 , In-Seon Lee 2
Affiliation  

Suaeda australis, Phragmites australis, Suaeda maritima, Suaeda glauca Bunge, and Limonium tetragonum in the Seocheon salt marsh on the west coast of the Korean Penincula were sampled in order to identify the endophytes inhabiting the roots. A total of 128 endophytic fungal isolates belonging to 31 different genera were identified using the fungal internal transcribed spacer (ITS) regions and the 5.8S ribosomal RNA gene. Fusarium, Paraconiothyrium and Alternaria were the most commonly isolated genera in the plant root samples. Various diversity indicators were used to assess the diversity of the isolated fungi. Pure cultures containing each of the 128 endophytic fungi, respectively, were tested for the plant growth-promoting abilities of the fungus on Waito-C rice germinals. The culture filtrate of the isolate Lt-1-3-3 significantly increased the growth of shoots compared to the shoots treated with the control. Lt-1-3-3 culture filtrate was analyzed and showed the presence of gibberellins (GA1 2.487 ng/ml, GA3 2.592 ng/ml, GA9 3.998, and GA24 6.191 ng/ml). The culture filtrate from the Lt-1-3-3 fungal isolate produced greater amounts of GA9 and GA24 than the wild-type Gibberella fujikuroi, a fungus known to produce large amounts of gibberellins. By the molecular analysis, fungal isolate Lt-1-3-3 was identified as Gibberella intermedia, with 100% similarity.

中文翻译:

耐盐植物的内生真菌:多样性和促进植物生长的能力。

对朝鲜半岛西海岸舒川盐沼中的碱蓬、芦苇、海碱蓬、碱蓬补血草进行了采样,以鉴定栖息在根部的内生菌。使用真菌内部转录间隔区 (ITS) 区域和 5.8S 核糖体 RNA 基因,共鉴定出属于 31 个不同属的 128 株内生真菌分离株。镰刀菌属、副孢菌属链格孢属是植物根样本中最常见的分离属。各种多样性指标用于评估分离真菌的多样性。分别对含有 128 种内生真菌的纯培养物进行了测试,以确定真菌对 Waito-C 水稻胚芽的植物生长促进能力。与用对照处理的枝条相比,分离物 Lt-1-3-3 的培养物滤液显着增加了枝条的生长。分析 Lt-1-3-3 培养滤液并显示存在赤霉素(GA1 2.487 ng/ml、GA3 2.592 ng/ml、GA9 3.998 和 GA24 6.191 ng/ml)。Lt-1-3-3 真菌分离物的培养滤液比野生型藤黑赤霉产生更多的 GA9 和 GA24,一种已知会产生大量赤霉素的真菌。经分子分析,分离真菌Lt-1-3-3被鉴定为Gibberella intermedia,相似度为100%。
更新日期:2021-09-15
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