The purpose of this study was to explore the hypouricemic effect in hyperuricemia mice of triterpenoid acids from Inonotus obliquus (TAIO), and decipher of the underlying xanthine oxidase inhibitory mechanism. Measurement of xanthine oxidase (XO) inhibitory activity was assayed. Organ indexes and serum biochemical indicators were measured in potassium oxonate-induced hyperuricemia mice. Studies showed that TAIO had the strong inhibitory effect on XO activity, and its inhibition type was mixed and reversible. In vivo, TAIO decreased efficiently uric acid level, hepatic XO, serum blood urea nitrogen activities in hyperuricemia mice. Indicating that TAIO may ameliorate kidney damage and relieve inflammation in hyperuricemic mice, and had the inhibitory effect on XO activity. Furthermore, eight triterpenoids were identified by Ultra performance liquid chromatography electrospray quadrupole time of flight mass spectrometry. These findings proved that triterpenoids from Inonotus obliquus would have potential biological characteristics and effect on controlling hyperuricemia and gout as an active supplement.

中文翻译：

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药用蘑菇桦褐孔菌 (Chaga) 中的三萜酸可缓解高尿酸血症小鼠的高尿酸血症和炎症：对黄嘌呤氧化酶活性的可能抑制作用

本研究旨在探讨桦褐孔菌三萜酸对高尿酸血症小鼠的降尿酸作用。（TAIO），并破译潜在的黄嘌呤氧化酶抑制机制。测定了黄嘌呤氧化酶(XO)抑制活性的测量。测定氧酸钾致高尿酸血症小鼠的脏器指标和血清生化指标。研究表明，TAIO对XO活性有较强的抑制作用，其抑制类型是混合的、可逆的。在体内，TAIO有效降低高尿酸血症小鼠的尿酸水平、肝XO、血清尿素氮活性。表明TAIO可改善高尿酸血症小鼠的肾脏损伤和减轻炎症，并对XO活性有抑制作用。此外，通过超高效液相色谱电喷雾四极杆飞行时间质谱法鉴定了八种三萜类化合物。这些发现证明了三萜类化合物桦褐孔菌作为活性补充剂具有潜在的生物学特性和控制高尿酸血症和痛风的作用。