The aim of the study was to investigate the effects of age and sex on the content of heavy metals (Pb, Cd, Zn and Cu) in the hair, liver and the longissimus lumborum muscle of roe deer (Capreolus capreolus L.). In addition, correlations between heavy metals in individual tested matrices were studied as well as an attempt was made to explain the reasons for their accumulation in specific research matrices. The levels of Zn and Cu were measured using atomic absorption spectroscopy (F-AAS). The levels of Pb and Cd were analysed by means of electrothermal atomic absorption spectroscopy (ET-AAS). In the own research, sex-related statistically significant differences in concentrations of analysed elements were confirmed. Higher levels of the elements were observed in samples collected from does (except for Cd in the liver samples of 6–7-month-old male fawns) but only in some cases, these values were statistically significantly higher. In some cases, age-related differences in the content of individual metals were also confirmed. Age-dependent increased Pb levels in the muscle and liver were reported in 6–7-month-old, <3–4-year-old, <5–6-year-old males. Similar relationships were confirmed for Zn in the bucks’ muscles. In the group of does, the increase in heavy metal concentrations with aging was observed for Zn in the muscle and for Pb in the liver. In the other cases, the trend of age-related level changes regarding heavy metals was not clearly demonstrated. Significant positive correlations between Cu and Cd were found for all samples, and significant negative correlations of Cu with Pb were found for the hair and liver. There was a negative correlation of Zn with Cu in all matrices. The study also revealed a negative correlation between Zn and Pb in muscle and a strong significant negative correlation between Zn and Cd in hair and in the liver.

该研究的目的是研究年龄和性别对狍毛发、肝脏和腰最长肌中重金属（ Pb 、Cd、Zn 和 Cu）含量的影响。L.）。此外，研究了各个测试矩阵中重金属之间的相关性，并试图解释它们在特定研究矩阵中积累的原因。使用原子吸收光谱 (F-AAS) 测量 Zn 和 Cu 的含量。通过电热原子吸收光谱 (ET-AAS) 分析 Pb 和 Cd 的含量。在自己的研究中，确认了分析元素浓度与性别相关的统计学显着差异。在从鹿采集的样本中观察到更高水平的元素（6-7 个月大的雄性小鹿肝脏样本中的 Cd 除外），但仅在某些情况下，这些值在统计学上显着更高。在某些情况下，还证实了与年龄相关的个别金属含量的差异。在 6-7 个月大、<3-4 岁、<5-6 岁的男性中，肌肉和肝脏中的 Pb 水平随年龄增加而增加。雄鹿肌肉中的锌也证实了类似的关系。在 does 组中，观察到肌肉中的 Zn 和肝脏中的 Pb 的重金属浓度随着年龄的增长而增加。在其他情况下，与年龄相关的重金属水平变化趋势并未得到明确证明。在所有样品中发现 Cu 和 Cd 之间存在显着正相关，在头发和肝脏中发现 Cu 和 Pb 之间存在显着负相关。在所有基质中 Zn 与 Cu 均呈负相关。该研究还揭示了肌肉中 Zn 和 Pb 之间的负相关关系，以及头发和肝脏中 Zn 和 Cd 之间的显着负相关关系。