The increasing use of gibberellic acid (GA₃) to promote fruit growth and yield has necessitated research into its trace level determination and estimation in harvested product. The phytohormone has increased the tomato yield (tonne ha⁻¹) up to 24.7% with uniform fruit shape, size colour and lustre. A fast, simple, high-throughput analytical method was standardised based on electrospray ionisation — liquid chromatography-tandem mass spectrometry (LC–MS/MS). The samples were extracted using acidified (1% formic acid) methanol. The method was validated as per the SANTE/12682/2019 guidelines. The limits of detection (LOD) and quantification (LOQ) were 0.01 and 0.05 mg kg⁻¹. The average recoveries at LOQ and higher levels were in the range of 86–108% with relative standard deviation (RSD) < 20%. The validated method was successfully applied under field condition by following first-order kinetics with half-lives (T_1/2) 1.76 days (recommended dose) and 1.99 days (double dose). The estimated pre-harvest intervals (PHIs) were 6 days (recommended dose) and 8 days (double dose). Studies on dietary risk assessment concluded that even after spray of GA₃ at recommended dose, the harvested produce (tomato) could be consumed safely.

越来越多地使用赤霉酸 (GA ₃ ) 来促进果实生长和产量，因此需要对其在收获产品中的痕量水平测定和估计进行研究。植物激素使番茄产量 (tonne ha ^-1 ) 增加了 24.7%，并具有均匀的果实形状、大小颜色和光泽。一种快速、简单、高通量的分析方法基于电喷雾电离 - 液相色谱 - 串联质谱 (LC-MS/MS) 进行了标准化。使用酸化（1% 甲酸）甲醇提取样品。该方法根据 SANTE/12682/2019 指南进行了验证。检测限 (LOD) 和定量 (LOQ) 分别为 0.01 和 0.05 mg kg ^-1. LOQ 和更高水平的平均回收率在 86-108% 的范围内，相对标准偏差 (RSD) < 20%。经验证的方法在现场条件下成功应用，遵循一级动力学，半衰期 (T _1/2 ) 1.76 天（推荐剂量）和 1.99 天（双倍剂量）。估计的收获前间隔 (PHI) 为 6 天（推荐剂量）和 8 天（双倍剂量）。饮食风险评估研究得出的结论是，即使在以推荐剂量喷洒 GA ₃后，收获的产品（番茄）也可以安全食用。