In this research, the Au nanoparticle was synthesized using a biosynthesized strategy by Mentha aquatic extract and characterized by Uv-vis spectroscopic method. The synthesized Au/NPs were used as a conductive mediator for the modification of the tramadol electrochemical sensor. The modified paste electrode with Au/NPs and 1-butyl-3-methylimidazolium tetrachloroborate (BMTCB) showed high catalytic activity for the determination of tramadol in aqueous solution. When compared to the bare electrode, the Au/NPs/BMTCB/CPE increased tramadol oxidation current by 8.8 times and lowered drug oxidation potential by 20 mV. The diffusion coefficient (D) ~ 2.55 × 10^− 5 cm²/s was calculated for electro-oxidation of tramadol at surface of Au/NPs/BMTCB/CPE. With a detection limit of 6.0 nM, the Au/NPs/BMTCB/CPE revealed a linear relationship between tramadol oxidation current and concentration in the range of 0.01–400.0 M.

在这项研究中，Au 纳米颗粒是使用薄荷水提取物的生物合成策略合成的，并通过紫外-可见光谱法表征。合成的 Au/NPs 用作导电介质，用于修饰曲马多电化学传感器。Au/NPs和1-丁基-3-甲基咪唑鎓四氯硼酸盐（BMTCB）修饰的糊状电极对水溶液中曲马多的测定显示出高催化活性。与裸电极相比，Au/NPs/BMTCB/CPE 将曲马多氧化电流提高了 8.8 倍，并将药物氧化电位降低了 20 mV。扩散系数 (D) ~ 2.55 × 10 ^{− 5} cm ²/s 计算曲马多在 Au/NPs/BMTCB/CPE 表面的电氧化。检测限为 6.0 nM，Au/NPs/BMTCB/CPE 揭示了曲马多氧化电流与浓度在 0.01-400.0 M 范围内的线性关系。