In the present work, the biodegradation of phenol by alginate immobilized Chlamydomonas reinhardtii cells was investigated. Immobilized Chlamydomonas reinhardtii could remove up to 1300 μmol/L of phenol within 10 days of cultivation. Metabolic activity was demonstrated by the extracellular release of catechol. Beads prepared at high concentrations of alginate (5–6% w/v) were found to protect microalgae against the strong inhibitory effects of phenol on the photosynthetic apparatus. Cells immobilized in beads of higher concentrations of alginate exhibited higher metabolic efficiencies compared to those prepared by lower alginate concentrations. Lower alginate concentrations (3–4% w/v) led to increased cell leakage, while the presence of phenol in the medium had the opposite effect in all alginate concentrations. Resuspension of immobilized microalgae in a medium containing a growth-promoting substrate, led to colony formation only on the external surface of alginate beads, indicating that acetic acid and consequently phenol, could not penetrate the internal of alginate beads. The significance of the work is that alginate immobilized Chlamydomonas substantially minimize the required volume of the aqueous medium and improve the economics and commercial application prospects of the process.

在目前的工作中，研究了藻酸盐固定的莱茵衣藻细胞对苯酚的生物降解。固定化莱茵衣藻培养 10 天内可去除高达 1300 μmol/L 的苯酚。儿茶酚的细胞外释放证明了代谢活性。发现以高浓度海藻酸盐 (5–6% w/v) 制备的珠子可以保护微藻免受苯酚对光合装置的强烈抑制作用。与通过较低藻酸盐浓度制备的细胞相比，固定在较高浓度藻酸盐珠中的细胞表现出更高的代谢效率。较低的海藻酸盐浓度 (3–4% w/v) 导致细胞渗漏增加，而培养基中苯酚的存在对所有海藻酸盐浓度都有相反的影响。将固定化微藻重新悬浮在含有促进生长的基质的培养基中，仅在藻酸盐珠的外表面上形成菌落，表明乙酸和苯酚不能渗透到海藻酸盐珠的内部。这项工作的意义在于固定藻酸盐衣藻大大减少了所需的水性介质体积，并提高了该方法的经济性和商业应用前景。