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Synergistic Theranostics of Magnetic Resonance Imaging and Photothermal Therapy of Breast Cancer Based on the Janus Nanostructures Fe3O4-Aushell-PEG
International Journal of Nanomedicine ( IF 8 ) Pub Date : 2021-09-16 , DOI: 10.2147/ijn.s322894 Xun Kang 1 , Tao Sun 1 , Liang Zhang 1 , Chunyu Zhou 1 , Zhongsheng Xu 1 , Mengmeng Du 1 , Shilin Xiao 1 , Yun Liu 1 , Mingfu Gong 1 , Dong Zhang 1
International Journal of Nanomedicine ( IF 8 ) Pub Date : 2021-09-16 , DOI: 10.2147/ijn.s322894 Xun Kang 1 , Tao Sun 1 , Liang Zhang 1 , Chunyu Zhou 1 , Zhongsheng Xu 1 , Mengmeng Du 1 , Shilin Xiao 1 , Yun Liu 1 , Mingfu Gong 1 , Dong Zhang 1
Affiliation
Background: Satisfactory prognosis of breast cancer (BC) is limited by difficulty in early diagnosis and insufficient treatment. The combination of molecular imaging and photothermal therapy (PTT) may provide a solution.
Methods: Fe3O4-Aushell nanoparticles (NPs) were prepared by thermal decomposition, seeded growth and galvanic replacement and were comprehensively characterized. After conjugated to PEG, NPs were used as MRI and PTT agents in vitro and in vivo.
Results: Fe3O4-Aushell NPs which had uniform Janus-like morphology were successfully synthesized. The Fe3O4 had a size of 18 ± 2.2 nm, and the Aushell had an outer diameter of 25 ± 3.3 nm and an inner diameter of 20 ± 2.9 nm. The NPs showed superparamagnetism, a saturation magnetization of 36 emu/g, and an optical absorption plateau from 700 to 808 nm. The Fe3O4-Aushell NPs were determined to possess good biocompatibility. After PEG coating, the zeta potential of NPs was changed from − 23.75 ± 1.37 mV to − 13.93 ± 0.55 mV, and the FTIR showed the characteristic C–O stretching vibration at 1113 cm− 1. The NPs’ MR imaging implied that the T2 can be shortened by Fe3O4-Aushell NPs in a concentration-dependent manner, and the Fe3O4-Aushell NPs coated with PEG at the molar ratio of 160 (PEG: NPs) showed the highest transverse relaxivity (r2) of 216 mM− 1s− 1. After irradiation at 0.65 W/cm2 for 5 min, all cells incubated with the Fe3O4-Aushell-PEG160 NPs (Fe: 30 ppm, Au: 70 ppm) died. After administrated intratumorally, Fe3O4-Aushell-PEG160 notably decreased the signal intensity of tumor in T2WI images. Under the same irradiation, the temperature of tumors injected with Fe3O4-Aushell-PEG160 quickly rose to 54.6°C, and the tumors shrank rapidly and were ablated in 6 days.
Conclusion: Fe3O4-Aushell-PEG NPs show good r2 and PTT performance and are promising synergistic theranostic agents of MRI and PTT for BC.
中文翻译：
基于 Janus 纳米结构 Fe3O4-Aushell-PEG 的乳腺癌磁共振成像和光热治疗协同治疗
背景：乳腺癌（BC）的令人满意的预后受到早期诊断困难和治疗不足的限制。分子成像和光热疗法（PTT）的结合可以提供一个解决方案。
方法：采用热分解、晶种生长和电置换制备Fe 3 O 4 -Au壳纳米粒子（NPs），并对其进行综合表征。与 PEG 缀合后，NP 在体外和体内用作 MRI 和 PTT 剂。
结果：成功合成了具有均匀Janus状形态的Fe 3 O 4 -Au壳纳米粒子。Fe 3 O 4尺寸为 18 ± 2.2 nm，Au壳的外径为 25 ± 3.3 nm，内径为 20 ± 2.9 nm。NPs 显示出超顺磁性，饱和磁化强度为 36 emu/g，光学吸收平台从 700 到 808 nm。Fe 3 O 4 -Au壳纳米颗粒被确定具有良好的生物相容性。PEG包覆后，纳米粒子的zeta电位从-23.75±1.37 mV变为-13.93±0.55 mV，FTIR在1113 cm -1处表现出特征性的C-O伸缩振动。NPs的MR成像暗示T 2可以被Fe 3 O 4 -Au壳缩短NPs 呈浓度依赖性，并且以160 (PEG: NPs) 的摩尔比涂有 PEG的 Fe 3 O 4 -Au壳NPs 显示出最高的横向弛豫率 ( r 2 ) 为 216 mM - 1 s - 1。在以0.65 W/cm 2照射5分钟后，用Fe 3 O 4 -Au壳-PEG160 NPs（Fe：30 ppm，Au：70 ppm）孵育的所有细胞都死亡。Fe 3 O 4 -Au壳-PEG160瘤内给药后显着降低肿瘤T 2信号强度WI 图像。在相同照射下，注射Fe 3 O 4 -Au壳-PEG160的肿瘤温度迅速升至54.6℃，肿瘤迅速缩小，6天后消融。
结论： Fe 3 O 4 -Au壳-PEG NPs具有良好的r 2和PTT性能，是一种很有前景的MRI和PTT协同治疗BC的药物。
更新日期：2021-09-16
Methods: Fe3O4-Aushell nanoparticles (NPs) were prepared by thermal decomposition, seeded growth and galvanic replacement and were comprehensively characterized. After conjugated to PEG, NPs were used as MRI and PTT agents in vitro and in vivo.
Results: Fe3O4-Aushell NPs which had uniform Janus-like morphology were successfully synthesized. The Fe3O4 had a size of 18 ± 2.2 nm, and the Aushell had an outer diameter of 25 ± 3.3 nm and an inner diameter of 20 ± 2.9 nm. The NPs showed superparamagnetism, a saturation magnetization of 36 emu/g, and an optical absorption plateau from 700 to 808 nm. The Fe3O4-Aushell NPs were determined to possess good biocompatibility. After PEG coating, the zeta potential of NPs was changed from − 23.75 ± 1.37 mV to − 13.93 ± 0.55 mV, and the FTIR showed the characteristic C–O stretching vibration at 1113 cm− 1. The NPs’ MR imaging implied that the T2 can be shortened by Fe3O4-Aushell NPs in a concentration-dependent manner, and the Fe3O4-Aushell NPs coated with PEG at the molar ratio of 160 (PEG: NPs) showed the highest transverse relaxivity (r2) of 216 mM− 1s− 1. After irradiation at 0.65 W/cm2 for 5 min, all cells incubated with the Fe3O4-Aushell-PEG160 NPs (Fe: 30 ppm, Au: 70 ppm) died. After administrated intratumorally, Fe3O4-Aushell-PEG160 notably decreased the signal intensity of tumor in T2WI images. Under the same irradiation, the temperature of tumors injected with Fe3O4-Aushell-PEG160 quickly rose to 54.6°C, and the tumors shrank rapidly and were ablated in 6 days.
Conclusion: Fe3O4-Aushell-PEG NPs show good r2 and PTT performance and are promising synergistic theranostic agents of MRI and PTT for BC.
中文翻译：
基于 Janus 纳米结构 Fe3O4-Aushell-PEG 的乳腺癌磁共振成像和光热治疗协同治疗
背景：乳腺癌（BC）的令人满意的预后受到早期诊断困难和治疗不足的限制。分子成像和光热疗法（PTT）的结合可以提供一个解决方案。
方法：采用热分解、晶种生长和电置换制备Fe 3 O 4 -Au壳纳米粒子（NPs），并对其进行综合表征。与 PEG 缀合后，NP 在体外和体内用作 MRI 和 PTT 剂。
结果：成功合成了具有均匀Janus状形态的Fe 3 O 4 -Au壳纳米粒子。Fe 3 O 4尺寸为 18 ± 2.2 nm，Au壳的外径为 25 ± 3.3 nm，内径为 20 ± 2.9 nm。NPs 显示出超顺磁性，饱和磁化强度为 36 emu/g，光学吸收平台从 700 到 808 nm。Fe 3 O 4 -Au壳纳米颗粒被确定具有良好的生物相容性。PEG包覆后，纳米粒子的zeta电位从-23.75±1.37 mV变为-13.93±0.55 mV，FTIR在1113 cm -1处表现出特征性的C-O伸缩振动。NPs的MR成像暗示T 2可以被Fe 3 O 4 -Au壳缩短NPs 呈浓度依赖性，并且以160 (PEG: NPs) 的摩尔比涂有 PEG的 Fe 3 O 4 -Au壳NPs 显示出最高的横向弛豫率 ( r 2 ) 为 216 mM - 1 s - 1。在以0.65 W/cm 2照射5分钟后，用Fe 3 O 4 -Au壳-PEG160 NPs（Fe：30 ppm，Au：70 ppm）孵育的所有细胞都死亡。Fe 3 O 4 -Au壳-PEG160瘤内给药后显着降低肿瘤T 2信号强度WI 图像。在相同照射下，注射Fe 3 O 4 -Au壳-PEG160的肿瘤温度迅速升至54.6℃，肿瘤迅速缩小，6天后消融。
结论： Fe 3 O 4 -Au壳-PEG NPs具有良好的r 2和PTT性能，是一种很有前景的MRI和PTT协同治疗BC的药物。
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