当前位置: X-MOL 学术Cancer Manage. Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Overexpression of the lncRNA AC012213.3 Promotes Proliferation, Migration and Invasion of Breast Cancer via RAD54B/PI3K/AKT Axis and is Associated with Worse Patient Prognosis
Cancer Management and Research ( IF 3.3 ) Pub Date : 2021-09-16 , DOI: 10.2147/cmar.s322195
Hongmei Zhong 1, 2, 3, 4 , Guilin Zeng 2, 3, 4 , Lang He 2, 3, 4
Affiliation  

Background: Long noncoding RNA (LncRNA) has been shown to mediate the development of human malignancies. However, data on its role in breast cancer remains scant. We aimed to evaluate the prognostic potential of lncRNAs in breast cancer.
Methods: We downloaded data on breast cancer patients from The Cancer Genome Atlas (TCGA) database. Tissues were obtained from The Fifth People’s Hospital of Chengdu. We then used the DESeq2 package to profile the expression of the lncRNAs between the patients and normal samples. Besides, we performed prognosis and survival analysis using survival tools in R package. We then assayed the role of the differentially expressed lncRNAs, AC012213.3 (ENSG00000266289), in cancer cell lines. Quantitative real-time PCR and Western blot analysis were performed to evaluate the expression of the gene in the cell lines and then assessed its role in the progression of breast cancer using cell proliferation (CCK8 and colony formation assays), migration, invasion (transwell and wound-healing assays) and apoptotic (flow cytometry) assays.
Results: Our data showed high expression of lncRNA AC012213.3 in breast cancer tissues and cell lines. The high expression of the AC012213.3 was associated with the worse prognosis and clinical features. Besides, in vitro assays demonstrated that downregulation of AC012213.3 suppresses the proliferation and invasion of breast cancer cells. Further analysis showed that RAD54B is a downstream AC012213.3 target gene and was upregulated in breast cancer. Interestingly, RAD54B expression was associated with shorter survival in breast cancer. In addition, AC012213.3 was shown to facilitate breast cancer progression through the RAD54B/PI3K/AKT axis.
Conclusion: Taken together, our data demonstrated that lncRNA AC012213.3 is upregulated in breast cancer and could enhance breast cancer progression through RAD54B/PI3K/AKT axis.



中文翻译:

lncRNA AC012213.3 的过表达通过 RAD54B/PI3K/AKT 轴促进乳腺癌的增殖、迁移和侵袭,并与更差的患者预后相关

背景:长链非编码 RNA (LncRNA) 已被证明可介导人类恶性肿瘤的发展。然而,关于其在乳腺癌中作用的数据仍然很少。我们旨在评估 lncRNA 在乳腺癌中的预后潜力。
方法:我们从癌症基因组图谱(TCGA)数据库下载了乳腺癌患者的数据。组织取自成都市第五人民医院。然后我们使用 DESeq2 包来分析患者和正常样本之间 lncRNA 的表达。此外,我们使用 R 包中的生存工具进行了预后和生存分析。然后,我们分析了差异表达的 lncRNA,AC012213.3 (ENSG00000266289) 在癌细胞系中的作用。进行定量实时 PCR 和蛋白质印迹分析以评估基因在细胞系中的表达,然后使用细胞增殖(CCK8 和集落形成测定)、迁移、侵袭(transwell 和伤口愈合测定)和凋亡(流式细胞术)测定。
结果:我们的数据显示lncRNA AC012213.3在乳腺癌组织和细胞系中的高表达。AC012213.3 的高表达与较差的预后和临床特征相关。此外,体外试验表明,AC012213.3 的下调抑制了乳腺癌细胞的增殖和侵袭。进一步分析表明,RAD54B 是下游 AC012213.3 靶基因,在乳腺癌中上调。有趣的是,RAD54B 表达与乳腺癌的较短生存期有关。此外,AC012213.3 显示通过 RAD54B/PI3K/AKT 轴促进乳腺癌进展。
结论:综上所述,我们的数据表明 lncRNA AC012213.3 在乳腺癌中上调,并且可以通过 RAD54B/PI3K/AKT 轴促进乳腺癌进展。

更新日期:2021-09-16
down
wechat
bug