Neoantigen vaccines are an immunotherapy strategy for treating cancer. The vaccine degrades quickly, so the strategy must include protection and precise targeting for immune cell stimulation. In this study, we engineered attenuated Salmonella typhimurium, which is highly infiltrative to tumors, to act as a carrier for Neoantigen peptide vaccine. Our system used a constitutive promoter vector, so that a single injection of Salmonella expressing Neoantigen could be used without requiring additional induction injections. In vivo experiments on bacteria-treated mice showed that Neoantigen expressed by the engineered carrier infiltrated tumors and resulted in suppressed tumor growth, higher survival rates and longer survival times, a relative increase of CD4 and CD8 T cells, and cytokine release. These results indicate that engineered Salmonella can be used as a carrier for Neoantigen immunotherapy.

中文翻译：

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表达新抗原的工程减毒鼠伤寒沙门氏菌具有抗癌作用

新抗原疫苗是一种治疗癌症的免疫治疗策略。疫苗会迅速降解，因此该策略必须包括保护和精确靶向免疫细胞刺激。在这项研究中，我们设计了对肿瘤具有高度浸润性的减毒鼠伤寒沙门氏菌作为新抗原肽疫苗的载体。我们的系统使用了组成型启动子载体，因此单次注射沙门氏菌无需额外的诱导注射即可使用表达新抗原。对经过细菌处理的小鼠进行的体内实验表明，工程载体表达的新抗原浸润肿瘤并导致肿瘤生长受到抑制、存活率更高、存活时间更长、CD4 和 CD8 T 细胞相对增加以及细胞因子释放。这些结果表明工程沙门氏菌可以用作新抗原免疫治疗的载体。