当前位置:
X-MOL 学术
›
ACS Sustain. Chem. Eng.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Ultrahigh Efficient FRET Ratiometric Fluorescence Biosensor for Visual Detection of Alkaline Phosphatase Activity and Its Inhibitor
ACS Sustainable Chemistry & Engineering ( IF 8.4 ) Pub Date : 2021-09-15 , DOI: 10.1021/acssuschemeng.1c03830 Yuanjin Zhan 1, 2 , Shuangting Yang 3 , Lifen Chen 3 , Yanbo Zeng 3 , Lei Li 3 , Zhenyu Lin 2 , Longhua Guo 3 , Wei Xu 1
ACS Sustainable Chemistry & Engineering ( IF 8.4 ) Pub Date : 2021-09-15 , DOI: 10.1021/acssuschemeng.1c03830 Yuanjin Zhan 1, 2 , Shuangting Yang 3 , Lifen Chen 3 , Yanbo Zeng 3 , Lei Li 3 , Zhenyu Lin 2 , Longhua Guo 3 , Wei Xu 1
Affiliation
Herein, a ratiometric fluorescence sensor based on the fluorescence resonance energy transfer (FRET) strategy was proposed to detect alkaline phosphatase (ALP) activity and its inhibitor (sodium vanadate, Na3VO4). The principle was that blue fluorescent nitrogen and sulfur co-doped carbon dots (N/S-CDs) were designed as energy donors, and ALP induced the formation of yellow fluorescent resultant 2,3-diaminophenazine (DPA) as an energy acceptor, causing the fluorescence to change from blue to yellow. Indeed, the FRET efficiency reached up to 93.2%, which is higher than most reported FRET strategies for sensing and imaging. After the recognition of ALP, the fluorescence intensity achieved a ratiometric change, accompanied by a significant transition in fluorescence color (Stokes wavelength shifted about 220 nm), which can achieve quantitative detection with a limit of detection being 0.396 U/L and visual qualitative analysis of ALP activity. Moreover, dual-emission self-correction ability can reduce external interference and improve the stability. The strong coordination ability between pyrophosphate (PPi) and Cu2+ and the efficient hydrolysis of PPi by ALP determined the specificity of the biosensor, and satisfactory results were obtained in the detection of clinical human serum samples. The proposed ratiometric fluorescence biosensor provides an alternative way for effective evaluation of ALP-related diseases, and it can also be used for the assessment of Cu2+, PPi, and Na3VO4 content and the screening of ALP inhibitors.
中文翻译:
用于视觉检测碱性磷酸酶活性及其抑制剂的超高效率 FRET 比率荧光生物传感器
在此,提出了一种基于荧光共振能量转移(FRET)策略的比率荧光传感器来检测碱性磷酸酶(ALP)活性及其抑制剂(钒酸钠、Na 3 VO 4)。其原理是设计蓝色荧光氮和硫共掺杂碳点(N/S-CDs)作为能量供体,ALP诱导形成黄色荧光所得2,3-二氨基吩嗪(DPA)作为能量受体,导致荧光由蓝色变为黄色。事实上,FRET 效率高达 93.2%,高于大多数报道的用于传感和成像的 FRET 策略。ALP识别后,荧光强度实现比例变化,伴随荧光颜色显着转变(斯托克斯波长偏移约220 nm),可实现定量检测,检出限为0.396 U/L,目视定性分析ALP 活动。此外,双发射自校正能力可以减少外部干扰,提高稳定性。2+和 ALP 对 PPi 的高效水解决定了生物传感器的特异性,在临床人血清样品的检测中取得了令人满意的结果。所提出的比率荧光生物传感器为ALP相关疾病的有效评估提供了一种替代方法,还可用于Cu 2+、PPi和Na 3 VO 4含量的评估以及ALP抑制剂的筛选。
更新日期:2021-09-27
中文翻译:
用于视觉检测碱性磷酸酶活性及其抑制剂的超高效率 FRET 比率荧光生物传感器
在此,提出了一种基于荧光共振能量转移(FRET)策略的比率荧光传感器来检测碱性磷酸酶(ALP)活性及其抑制剂(钒酸钠、Na 3 VO 4)。其原理是设计蓝色荧光氮和硫共掺杂碳点(N/S-CDs)作为能量供体,ALP诱导形成黄色荧光所得2,3-二氨基吩嗪(DPA)作为能量受体,导致荧光由蓝色变为黄色。事实上,FRET 效率高达 93.2%,高于大多数报道的用于传感和成像的 FRET 策略。ALP识别后,荧光强度实现比例变化,伴随荧光颜色显着转变(斯托克斯波长偏移约220 nm),可实现定量检测,检出限为0.396 U/L,目视定性分析ALP 活动。此外,双发射自校正能力可以减少外部干扰,提高稳定性。2+和 ALP 对 PPi 的高效水解决定了生物传感器的特异性,在临床人血清样品的检测中取得了令人满意的结果。所提出的比率荧光生物传感器为ALP相关疾病的有效评估提供了一种替代方法,还可用于Cu 2+、PPi和Na 3 VO 4含量的评估以及ALP抑制剂的筛选。