Biomonitoring with living organisms is most often used in order to gather quick information about environmental quality – for example the level of heavy metals polluting the air. Experiments are not often performed when the bioindicator is exposed to pollutants over the long time (6 months and more) because this period required to obtain credible results and the risk not yet studied of physiological changes and of degeneration of the living material which would undoubtedly affect the biomonitoring results. The aim of study was to evaluate the physiological condition of Pleurozium schreberi moss species subjected to continuous exposure for a year, using the moss-bag method, under variable environmental conditions, including atmospheric aerosol contaminated by selected analytes: Cu, Zn, Cd, Hg, Pb determined by flame atomic absorption spectrometer. AMA apparatus was used to measure mercury. The survival of the moss during exposure was assessed based on the measurement of chlorophyll content. As a result of the research, it was found that the concentrations of metals in mosses increased depending on the exposure time and the certain element. It was also found that mosses, despite the long period of exposure to environmental factors and the decreasing content of chlorophyll, still revealed the features of a living organism during the study and can be used in long-term biomonitoring of the environment. It was also proposed to correct the period and duration of P. schreberi exposure to air pollution in relation to the previous literature and recommend the period of 3–6 months.

对活生物体进行生物监测最常用于收集有关环境质量的快速信息——例如污染空气的重金属水平。当生物指示剂长时间（6 个月及以上）暴露在污染物中时，通常不进行实验，因为这段时间需要获得可靠的结果，而生理变化和生物材料退化的风险尚未研究，这无疑会影响生物监测结果。研究的目的是评估平菇的生理状况使用苔藓袋法在可变环境条件下连续暴露一年的苔藓物种，包括被选定分析物污染的大气气溶胶：通过火焰原子吸收光谱仪测定的铜、锌、镉、汞、铅。AMA 仪器用于测量汞。基于叶绿素含量的测量评估苔藓在暴露期间的存活率。研究结果发现，苔藓中的金属浓度随暴露时间和特定元素而增加。研究还发现，苔藓虽然长期暴露于环境因素，叶绿素含量不断下降，但在研究过程中仍能揭示生物体的特征，可用于环境的长期生物监测。P. schreberi暴露于空气污染相对于以前的文献和建议的时间为 3-6 个月。