Two different simple, accurate, and precise UV spectrophotometric methods have been developed for the estimation of erlotinib hydrochloride in bulk and tablet forms by the zero-order (method I) and the zero-order AUC (method II). The drug was dissolved in a phosphate buffer solution (pH 7.4), and at 228.20 nm the zero-order (method I) and at 224.20–230.20 nm the zero-order AUC (method II) were developed. For both methods, erlotinib has linearity in the concentration range 10–70 μg/mL, with a correlation coefficient R² > 0.99. Both methods showed good reproducibility and recovery with %RSD less than 2 and an accuracy of 98.54–101.39 and 98.08–99.83%, respectively. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.16, 3.51, and 21.41, 64.90 μg/mL, respectively, for both methods.

已经开发了两种不同的简单、准确和精确的紫外分光光度法，用于通过零级（方法 I）和零级 AUC（方法 II）估算散装和片剂形式的盐酸厄洛替尼。将药物溶解在磷酸盐缓冲溶液 (pH 7.4) 中，在 228.20 nm 处产生零级 AUC（方法 I），在 224.20–230.20 nm 处产生零级 AUC（方法 II）。对于这两种方法，厄洛替尼在 10–70 μg/mL 的浓度范围内具有线性，相关系数 R ² > 0.99。两种方法均显示出良好的重现性和回收率，%RSD 小于 2，准确度分别为 98.54–101.39 和 98.08–99.83%。两种方法的检测限 (LOD) 和定量限 (LOQ) 分别为 1.16、3.51 和 21.41、64.90 μg/mL。