An inclusion complex was formed with methyl-β-cyclodextrin (Me-β-CD) and the smallest unit of the macrocyclic family of bathocuproine, 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline. With this newly formed fluorescent probe, the quenching effect of heavy metals was investigated. Spectral characterization of the complex was performed by UV-visible and fluorescence spectroscopy techniques. The complex formation constant (K_b), Gibbs free energy (ΔG⁰), and approximate quenching activation energy (E_a) values were calculated. Lifetime (τ), bimolecular quenching rate constant (k_q) values, and diffusion rate constant (k_d) calculations were made, and the mechanism of quenching by the effect of heavy metals was clarified.

由甲基-β-环糊精 (Me-β-CD) 和浴铜灵大环家族的最小单元 2,9-二甲基-4,7-二苯基-1,10-菲咯啉形成包合物。使用这种新形成的荧光探针，研究了重金属的猝灭效应。通过紫外-可见光和荧光光谱技术对复合物进行光谱表征。计算复合物形成常数 ( K _b )、吉布斯自由能 (Δ G ⁰ ) 和近似淬火活化能 ( E _a ) 值。寿命 (τ)、双分子淬灭速率常数 ( k _q ) 值和扩散速率常数 ( k _d)进行了计算，阐明了重金属作用淬火的机理。