当前位置: X-MOL 学术Biotechnol. Biofuels › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Improving sustainable hydrogen production from green waste: [FeFe]-hydrogenases quantitative gene expression RT-qPCR analysis in presence of autochthonous consortia
Biotechnology for Biofuels ( IF 6.3 ) Pub Date : 2021-09-16 , DOI: 10.1186/s13068-021-02028-3
M Arizzi 1, 2 , S Morra 1, 3 , G Gilardi 1 , M Pugliese 4, 5 , M L Gullino 4, 5 , F Valetti 1
Affiliation  

Bio-hydrogen production via dark fermentation of low-value waste is a potent and simple mean of recovering energy, maximising the harvesting of reducing equivalents to produce the cleanest fuel amongst renewables. Following several position papers from companies and public bodies, the hydrogen economy is regaining interest, especially in combination with circular economy and the environmental benefits of short local supply chains, aiming at zero net emission of greenhouse gases (GHG). The biomasses attracting the largest interest are agricultural and urban green wastes (pruning of trees, collected leaves, grass clippings from public parks and boulevards), which are usually employed in compost production, with some concerns over the GHG emission during the process. Here, an alternative application of green wastes, low-value compost and intermediate products (partially composted but unsuitable for completing the process) is studied, pointing at the autochthonous microbial consortium as an already selected source of implementation for biomass degradation and hydrogen production. The biocatalysts investigated as mainly relevant for hydrogen production were the [FeFe]-hydrogenases expressed in Clostridia, given their very high turnover rates. Bio-hydrogen accumulation was related to the modulation of gene expression of multiple [FeFe]-hydrogenases from two strains (Clostridium beijerinckii AM2 and Clostridium tyrobutyricum AM6) isolated from the same waste. Reverse Transcriptase quantitative PCR (RT-qPCR) was applied over a period of 288 h and the RT-qPCR results showed that C. beijerinckii AM2 prevailed over C. tyrobutyricum AM6 and a high expression modulation of the 6 different [FeFe]-hydrogenase genes of C. beijerinckii in the first 23 h was observed, sustaining cumulative hydrogen production of 0.6 to 1.2 ml H2/g VS (volatile solids). These results are promising in terms of hydrogen yields, given that no pre-treatment was applied, and suggested a complex cellular regulation, linking the performance of dark fermentation with key functional genes involved in bio-H2 production in presence of the autochthonous consortium, with different roles, time, and mode of expression of the involved hydrogenases. An applicative outcome of the hydrogenases genes quantitative expression analysis can be foreseen in optimising (on the basis of the acquired functional data) hydrogen production from a nutrient-poor green waste and/or low added value compost, in a perspective of circular bioeconomy.

中文翻译:

改善绿色废物的可持续制氢:[FeFe]-氢化酶定量基因表达 RT-qPCR 分析在本土财团存在下

通过低价值废物的暗发酵生产生物氢是一种有效且简单的能量回收方法,可最大限度地减少还原当量的收获,以生产可再生能源中最清洁的燃料。根据公司和公共机构的几份立场文件,氢经济正在重新引起人们的兴趣,特别是与循环经济和当地短供应链的环境效益相结合,旨在实现温室气体 (GHG) 的零净排放。最受关注的生物质是农业和城市绿色废物(树木的修剪、收集的树叶、公园和林荫大道上的草屑),它们通常用于堆肥生产,但对过程中的温室气体排放存在一些担忧。在这里,绿色废物的替代应用,对低价值堆肥和中间产品(部分堆肥但不适合完成该过程)进行了研究,指出本地微生物群落已被选为生物质降解和制氢的实施来源。被研究的主要与产氢相关的生物催化剂是在梭菌中表达的 [FeFe]-氢化酶,因为它们的周转率非常高。生物氢积累与从同一废物中分离出的两种菌株(拜氏梭菌 AM2 和酪丁酸梭菌 AM6)的多种 [FeFe]-氢化酶的基因表达调节有关。逆转录酶定量 PCR (RT-qPCR) 应用了 288 小时,RT-qPCR 结果显示 C. beijerinckii AM2 优于 C. 在最初的 23 小时内观察到酪丁酸 AM6 和 C. beijerinckii 的 6 种不同 [FeFe]-氢化酶基因的高表达调节,维持 0.6 至 1.2 ml H2/g VS(挥发性固体)的累积氢气产量。鉴于没有应用预处理,这些结果在氢气产量方面很有希望,并表明存在复杂的细胞调节,将暗发酵的性能与在本土聚生体存在下参与生物 H2 生产的关键功能基因联系起来,与所涉及的氢化酶的不同作用、时间和表达方式。可以预见氢化酶基因定量表达分析的应用结果可用于优化(基于获得的功能数据)从营养贫乏的绿色废物和/或低附加值堆肥中生产氢气,
更新日期:2021-09-16
down
wechat
bug