To confirm the distribution of the allopolyploid and ancestral species in the Matsukawaura Lagoon in a hybrid zone between Neopyropia yezoensis and Neopyropia tenera, molecular marker analyses and culture experiments were performed using collected blades. All the blades examined, except for one blade identified as N. tenera, exhibited nuclear genotypes of both species, but only the chloroplast haplotype of N. yezoensis. In addition, four conchocelis strains were isolated from each of the blades, and they contained genotypes of both N. yezoensis and N. tenera; a conchocelis strain from the sole N. tenera blade was also isolated. Using the conchocelis strains, F₁ blades that were produced from archeospores were obtained under laboratory culture and examined by nuclear DNA markers. These results indicated that the collected blades and the examined F₁ blades, except for the single N. tenera blade, were allodiploids and that the four conchocelis strains could be allotetraploids derived from interspecific hybridization between N. yezoensis as the maternal strain and N. tenera as the paternal strain. Therefore, most of the Neopyropia blades growing in the Matsukawaura Lagoon are presumed to be from allopolyploid lineages derived from interspecific hybridization. Furthermore, a growth comparison between the allopolyploid strain and the N. tenera strain was carried out under identical culture conditions. The rate of increase in blade length was significantly higher in the allopolyploid hybrid than in the pure N. tenera. The results suggest that further exploration of allopolyploidy in the potential hybrid zones will be needed not only for the investigation of speciation mechanisms in the bladed Bangiales but also for the genetic improvement of the marine crop.

中文翻译：

---

在松川浦泻湖（日本）内的条状新火龙和新火龙（Bangiales，Rhodophyta）之间的混合区中稳定的异源多倍体及其与物种形成机制和作物改良的相关性

为确认条状新火龙和新火龙混杂区的松川浦泻湖中异源多倍体和祖先物种的分布，使用收集的叶片进行分子标记分析和培养实验。除了一个被标识为N 的刀片外，所有的刀片都被检查了。 tenera，显示了两个物种的核基因型，但只有N的叶绿体单倍型。 条斑紫菜。此外，从每个叶片中分离出 4 个 conchocelis 菌株，它们包含两种N 的基因型。 条藻和N . 特内拉; 来自唯一N的 conchocelis 菌株。 特内拉刀片也被隔离。使用螺壳菌菌株，在实验室培养下获得由古孢子产生的F ₁叶片，并通过核 DNA 标记进行检查。这些结果表明收集的叶片和检查的 F ₁叶片，除了单个N。 tenera Blade 是异源二倍体，并且这四个 conchocelis 菌株可能是来自N之间的种间杂交的异源四倍体。 yezoensis作为母株和N . tenera作为父系菌株。因此，大多数在松川浦泻湖中生长的Neopyropia叶片被推测为来自种间杂交的异源多倍体谱系。此外，异源多倍体菌株和N之间的生长比较。 tenera菌株在相同的培养条件下进行。异源多倍体杂种的叶片长度增加率明显高于纯N。 特内拉。结果表明，不仅需要对有叶片的 Bangiales 物种形成机制进行研究，而且还需要对海洋作物的遗传改良，进一步探索潜在杂交区的异源多倍体。