Transposons are mobile genetic elements in the genome. The piggyBac (PB) transposon system is increasingly being used for stem cell research due to its high transposition efficiency and seamless excision capacity. Over the past few decades, forward genetic screens based on PB transposons have been successfully established to identify genes associated with drug resistance and stem cell-related characteristics. Moreover, PB transposon is regarded as a promising gene therapy vector and has been used in some clinically relevant stem cells. Here, we review the recent progress on the basic biology of PB, highlight its applications in current stem cell research, and discuss its advantages and challenges.

中文翻译：

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PiggyBac 转座子在干细胞基因组操作中的应用

转座子是基因组中的可移动遗传元件。在piggyBac转（PB）转座子系统越来越多地用于干细胞的研究，由于其高效率换位和无缝切除能力。在过去的几十年中，已经成功建立了基于 PB 转座子的正向遗传筛选，以识别与耐药性和干细胞相关特征相关的基因。此外，PB转座子被认为是一种很有前途的基因治疗载体，并已用于一些临床相关的干细胞。在这里，我们回顾了 PB 基础生物学的最新进展，重点介绍了其在当前干细胞研究中的应用，并讨论了其优势和挑战。