Salmonella infections in eggs with increasing morbidity and mortality exhibit worldwide prevalence. The present study intends to evaluate the efficacy of Lactobacillus reuteri Lb11 (L. reuteri Lb11, isolated from chicken intestinal tract) in inhibiting the growth of multi-drug resistant (MDR) Salmonella Enteritidis SE05 (obtained from egg content). The cell-free cell lysates (CFCL) of L. reuteri Lb11 obtained by the agar spot test performed well on inhibition of the MDR (Multi-Drug Resistant) Salmonella Enteritidis SE05, The heat-inactivated (HI) fraction of L. reuteri Lb11 showed no inhibition activity. By co-culturing with L. reuteri Lb11 in vitro, the growth of S. Enteritidis SE05 decreased along with time, while, the pH value decreased significantly. Furthermore, In order to evaluate the mechanism of action of CFCL of L.reuteri Lb11, the genes related to the transcription level of AcrAB-TolC efflux pump, outer membrane protein OMPs genes and drug resistance genes have been quantified by real-time PCR, when the S. Enteritidis was SE05 exposed to the CFCL of L. reuteri Lb11 (1 × 10¹² CFU/mL). Almost all of the AcrAB-TolC efflux pump genes, outer membrane protein genes and antibiotic resistance genes were down-regulated. Especially, the level of ramA, tetA and tetB genes were down-regulated -20.77, -15.85 and -12.42 folds, respectively. L. reuteri Lb11 can effectively prevent the formation of efflux pump to inhibit the production of multidrug-resistant Salmonella Enteritidis in eggs.

随着发病率和死亡率的增加，鸡蛋中的沙门氏菌感染在世界范围内流行。本研究旨在评估罗伊氏乳杆菌Lb11（罗伊氏乳杆菌Lb11，从鸡肠道分离）抑制多重耐药（MDR）肠炎沙门氏菌 SE05（从鸡蛋中获得）生长的功效。无细胞的细胞裂解物的（CFCL）罗伊氏由琼脂斑试验得到LB11上抑制MDR（多药耐药）的表现良好沙门氏菌肠炎SE05，所述的热灭活（HI）分数罗伊氏LB11无抑制活性。通过与罗伊氏乳杆菌共培养LB11体外，生长S.肠炎SE05随时间而降低沿，而将pH值显著下降。此外，为了评估罗伊氏乳杆菌Lb11 CFCL的作用机制，实时定量了与Acr AB- Tol C外排泵转录水平相关的基因、外膜蛋白OMPs基因和耐药基因。 PCR，当S.肠炎是SE05暴露于的CFCL罗伊氏乳杆菌LB11（1×10 ¹² CFU/毫升）。几乎所有的 AcrAB-TolC 外排泵基因、外膜蛋白基因和抗生素抗性基因都被下调。特别是，ramA、tetA和tetB基因的水平分别下调-20.77、-15.85和-12.42倍。罗伊氏乳杆菌Lb11能有效阻止外排泵的形成，抑制鸡蛋中多重耐药肠炎沙门氏菌的产生。