Ethnopharmacological relevance

Since ancient times, herbal medicines have been applied in the treatment of cancer. Tea, derivative from the dried leaves of Camellia sinensis (L.) Kuntze plant is the most popular beverage globally after water and is available in various forms. Green tea has been expansively investigated for its beneficial properties of cancer prevention and therapy. The goal of the research: The current study was conducted to evaluate the hepaprotective character of methanolic green tea extract and its mechanism of action contrary to thioacetamide (TAA)-produced liver fibrosis of Sprague Dawley rats.

Materials and Methods

Thirty rodents were equally placed in 5 clusters including normal control, TAA group as a positive control, silymarin as standard drug control, and treatment groups consisting of high dose and a low dose Camellia sinensis. Rats in experimental clusters by mouth fed with C. sinensis at 250 mg/kg or 500 mg/kg daily for 2 months. After 60 days, all rats were sacrificed. Blood specimens were gathered for liver biochemical examination. Livers of all groups were dissected out and subjected to histopathological examination through the Hematoxylin and Eosin stain, Masson trichrome, and immunohistochemistry stains (PCNA). Liver tissue homogenate was also analyzed for antioxidant activity parameters.

Results

Gross morphological examination showed a regular liver architecture in C. sinensis fed collections compared to the TAA sets. Histology of rat’s liver fed with C. sinensis showed an important decrease in the liver index with hepatic cells propagation, mild cellular injury, and immunostaining showed significant down-expression of proliferating cell nuclear antigen (PCNA). TAA produced liver fibrosis through a significant increase in serum alanine transferase, aspartate aminotransferase, alkaline phosphatase, and bilirubin. Total protein and albumin also decreased in the TAA group. Moreover, the reduction of antioxidant enzyme activity including superoxide dismutase and catalase as well as the increase in malondialdehyde was detected in the TAA control group. Meanwhile, an abnormal level of liver biochemical parameters was restored closer to the normal levels in serum of the C. sinensis-fed clusters. In addition, C. sinensis fed assemblies showed elevated antioxidative enzymes activity with a reduction in malondialdehyde level comparable to the levels in silymarin-treated rats.

Conclusions

Green tea potentially inhibited the progression of liver cirrhosis, down -regulation of PCNA proliferation, prevented oxidation of hepatocytes, recovered SOD and CAT enzymes, condensed MDA and reduced cellular inflammation.

中文翻译：

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绿茶甲醇提取物对硫代乙酰胺诱导的斯普拉格道利大鼠肝损伤的保护作用

民族药理学相关性

自古以来，草药就被应用于治疗癌症。茶是从 Camellia sinensis (L.) Kuntze 植物的干叶中提取的，是继水之后全球最受欢迎的饮料，并且有多种形式。绿茶因其预防和治疗癌症的有益特性而被广泛研究。研究目的：本研究旨在评估绿茶甲醇提取物的肝脏保护特性及其对抗硫代乙酰胺 (TAA) 引起的 Sprague Dawley 大鼠肝纤维化的作用机制。

材料和方法

将30只啮齿动物平均分为5组，包括正常对照、TAA组作为阳性对照、水飞蓟素作为标准药物对照、以及由高剂量和低剂量茶树组成的治疗组。实验组中的大鼠每天口服250 mg/kg或500 mg/kg中华茶树，持续2个月。60天后，处死所有大鼠。采集血液标本进行肝脏生化检查。解剖所有组的肝脏并通过苏木精和曙红染色、Masson三色和免疫组织化学染色(PCNA)进行组织病理学检查。还分析了肝组织匀浆的抗氧化活性参数。

结果

总体形态学检查显示，与 TAA 组相比，中华线虫饲养组的肝脏结构规则。饲喂中华线虫的大鼠肝脏组织学显示，随着肝细胞增殖，肝脏指数显着下降，细胞轻度损伤，免疫染色显示增殖细胞核抗原（PCNA）表达显着下调。TAA通过显着增加血清丙氨酸转移酶、天冬氨酸转氨酶、碱性磷酸酶和胆红素而导致肝纤维化。TAA 组的总蛋白和白蛋白也有所下降。此外，在TAA对照组中检测到抗氧化酶活性（包括超氧化物歧化酶和过氧化氢酶）降低以及丙二醛增加。同时，饲喂中华中华线虫簇的肝脏生化参数的异常水平恢复到接近正常水平。此外，饲喂中华水飞蓟的组合显示出抗氧化酶活性升高，丙二醛水平降低，与水飞蓟素处理的大鼠水平相当。

结论

绿茶可能抑制肝硬化的进展、下调 PCNA 增殖、防止肝细胞氧化、恢复 SOD 和 CAT 酶、浓缩 MDA 并减少细胞炎症。