Endophytic fungi play an important role in plant survival and reproduction, but the role of their metabolites in plant growth and immunity, as well as in crop quality formation, is poorly understood. Zhinengcong (ZNC) is a crude ethanol extract from the endophytic fungus Paecilomyces variotii, and previous studies have shown that it can improve the growth and immunity in Arabidopsis thaliana. The aim of the study was to reveal the trade-off balance between plant growth and immunity by evaluating the mechanisms of ZNC on potato growth, yield, and priming immunity against the oomycete Phytophthora infestans indoors and in the field. ZNC maintained a good balance between plant growth and resistance against P. infestans with high activity. It induced the reactive oxygen species (ROS) production, promoted plant growth, yield and quality parameters, enhanced the expression of indoleacetic acid (IAA) related genes, and increased the absorption of nitrogen from the soil. Moreover, the plant endophytic fungus extract ZNC stimulated the pathogen-associated molecular pattern (PAMP) triggered immunity (PTI) pathway and contributed to the ZNC-mediated defense response. Two years of field trials have shown that irrigation with ZNC at one of two optimal concentrations of 1 or 10ng/ml could significantly increase the output by 18.83% or more. The quality of potato tubers was also greatly improved, in which the contents of vitamin C, protein, and starch were significantly increased, especially the sugar content was increased by 125%. Spray application of ZNC onto potato plants significantly reduced the occurrence of potato blight disease with 66.49% of control efficacy at 200ng/ml and increased the potato yield by 66.68% or more in the field. In summary, plant endophytic fungus extract ZNC promoted potato immunity, yield, and quality and presented excellent potential in agricultural applications.

内生真菌在植物生存和繁殖中发挥重要作用，但其代谢物在植物生长和免疫以及作物品质形成中的作用却知之甚少。智能聪 (ZNC) 是一种从内生真菌中提取的粗乙醇提取物拟青霉, 之前的研究表明它可以提高生长和免疫力 拟南芥. 本研究的目的是通过评估 ZNC 对马铃薯生长、产量和针对卵菌的启动免疫的机制，揭示植物生长和免疫之间的权衡平衡。致病疫霉室内和野外。ZNC在植物生长和抗性之间保持了良好的平衡致病疫霉具有高活性。它诱导活性氧 (ROS) 产生，促进植物生长、产量和质量参数，增强吲哚乙酸 (IAA) 相关基因的表达，并增加土壤对氮的吸收。此外，植物内生真菌提取物 ZNC 刺激病原体相关分子模式 (PAMP) 触发免疫 (PTI) 途径，并有助于 ZNC 介导的防御反应。两年的田间试验表明，以 1 或 10 ng/ml 的两个最佳浓度之一使用 ZNC 进行灌溉可以显着增加产量 18.83% 或更多。马铃薯块茎的品质也大大提高，其中维生素C、蛋白质、淀粉含量显着提高，特别是含糖量提高了125%。在马铃薯植株上喷施ZNC可显着降低马铃薯疫病的发生，在200ng/ml时防效达66.49%，大田马铃薯产量提高66.68%以上。总之，植物内生真菌提取物 ZNC 促进了马铃薯的免疫、产量和质量，并在农业应用中具有极好的潜力。