Persistent hyperglycaemia and scopolamine were used to inflict amnesia in rats. Chronic hyperglycaemia causes metabolic impairment, neuronal dysfunction and oxidative stress causing cognitive impairment. This study aimed to determine anti amnesic activities of vitamin D, epalrestat and their combination against diabetes and scopolamine induced cognitive dysfunction. A total of eighty-eight Wistar albino rats, eleven groups, and 8 rats/Gr., were used. Type 2 diabetes mellitus was induced in all groups, except Gr.1 which was treated with 2 ml normal saline. Gr. 2 to 11 by feeding high fat diet for 28 days followed by single dose streptozotocin 35 mg/kg i.p. Hyperglycemic rats were screened with blood sugar level > 200 mg/dL. Gr. 2 rats were treated with only streptozotocin and Gr. 3 to 6 were treated with streptozotocin and test drugs donepezil 1 mg/kg, vitamin D, 27 mcg/kg, epalrestat 57 mg/kg, vitamin D + epalrestat, per oral, respectively. Gr. 7 rats were treated with only streptozotocin + scopolamine and all others from Gr. 8 to 11 were treated with streptozotocin + scopolamine and donepezil, vitamin D, epalrestat, vitamin D + epalrestat respectively. The gold standard behavioural tests were conducted by using Morris water maze and passive avoidance paradigms after 30–60 min of inj. scopolamine, 0.5 mg/kg, intra-peritoneal. Hippocampal tissue was taken for histopathological and biochemical evaluation. Rats treated with donepezil, vitamin D, epalrestat and vitamin D + epalrestat showed significant improvement in behavioural, biochemical and histopathological parameters as compared to streptozotocin and (streptozotocin + scopolamine) treated rats. This study underscores cognition enhancing abilities of vitamin D and epalrestat, and their combination in diabetic rats with and without scopolamine.

持续的高血糖症和东莨菪碱被用来在大鼠中造成健忘症。慢性高血糖会导致代谢障碍、神经元功能障碍和氧化应激导致认知障碍。本研究旨在确定维生素 D、依帕司他及其组合对糖尿病和东莨菪碱诱导的认知功能障碍的抗遗忘活性。共八十八只Wistar白化病大鼠, 使用了 11 组和 8 只大鼠/Gr.。除Gr.1用2ml生理盐水治疗外，所有组均诱发2型糖尿病。格。2 至 11 喂高脂肪饮食 28 天，然后单剂量链脲佐菌素 35 mg/kg ip 筛选血糖水平 > 200 mg/dL 的高血糖大鼠。格。2只大鼠仅用链脲佐菌素和Gr处理。3 至 6 名患者分别口服链脲佐菌素和试验药物多奈哌齐 1 mg/kg、维生素 D 27 mcg/kg、依帕司他 57 mg/kg、维生素 D + 依帕司他。格。7 只大鼠仅用链脲佐菌素 + 东莨菪碱和来自 Gr 的所有其他大鼠进行治疗。8～11例分别用链脲佐菌素+东莨菪碱和多奈哌齐、维生素D、依帕司他、维生素D+依帕司他治疗。在注射 30-60 分钟后，通过使用莫里斯水迷宫和被动回避范式进行黄金标准行为测试。东莨菪碱，0.5 mg/kg，腹膜内。取海马组织进行组织病理学和生化评估。与链脲佐菌素和（链脲佐菌素 + 东莨菪碱）治疗的大鼠相比，用多奈哌齐、维生素 D、依帕司他和维生素 D + 依帕司他治疗的大鼠表现出行为、生化和组织病理学参数的显着改善。这项研究强调了维生素 D 和依帕司他的认知增强能力，以及它们在有和没有东莨菪碱的糖尿病大鼠中的组合。与链脲佐菌素和（链脲佐菌素 + 东莨菪碱）治疗的大鼠相比，依帕司他和维生素 D + 依帕司他在行为、生化和组织病理学参数方面表现出显着改善。这项研究强调了维生素 D 和依帕司他的认知增强能力，以及它们在有和没有东莨菪碱的糖尿病大鼠中的组合。与链脲佐菌素和（链脲佐菌素 + 东莨菪碱）治疗的大鼠相比，依帕司他和维生素 D + 依帕司他在行为、生化和组织病理学参数方面表现出显着改善。这项研究强调了维生素 D 和依帕司他的认知增强能力，以及它们在有和没有东莨菪碱的糖尿病大鼠中的组合。