In order to develop a more sensitive and reliable method for detection of serum antibodies against Mycoplasma hyopneumoniae infection in pigs, six recombinant proteins of M. hyopneumoniae (P102, P95, P46, P97 like, Lppt, and hypothetical P987) were used for the standardization of an indirect enzyme-linked immunosorbent assay (ELISA). The proteins were evaluated against 50 sera of the specific pathogen-free and 50 sera of pigs with lesions suggestive of infection. The sensitivity was 88%, 86%, 78%, 74%, 66%, and 60% for the proteins P102, P95, P46, P97 like, Lppt, and hypothetical protein P987, respectively. Moreover, the proteins were used to establish the seroprevalence in two different commercial herds (254 sera pigs from farm considered free of M. hyopneumoniae and 246 from farm with clinical signs of enzootic pneumonia and positive serology for M. hyopneumoniae) and the positive rate was 65.2% for P95, 54.6% for P102, 40.2% for P46, 37.2% for P97 like, 17.4% for the hypothetical P987, and 14% for Lppt protein. In addition, the ELISA with six recombinant proteins was compared to commercial HerdCheck kit using 118 random pig sera samples and the results showed that ELISA with recombinant proteins were more sensitive than the commercial test. These data show that the recombinant proteins P95 and P102 are potential targets to be used in diagnostic tests to detect antibodies against M. hyopneumoniae. Although more studies are necessary, this study provides insights that these recombinant proteins can be useful in epidemiological investigations and as potential biomarkers in differentiating infected animals from those vaccinated.

为了开发一种更灵敏可靠的猪肺炎支原体感染血清抗体检测方法，使用六种猪肺炎支原体重组蛋白（P102、P95、P46、P97 like、Lppt 和假设的 P987）进行标准化间接酶联免疫吸附试验（ELISA）。针对 50 种无特定病原体的血清和 50 种具有提示感染病变的猪的血清对蛋白质进行了评估。蛋白质 P102、P95、P46、P97 样、Lppt 和假设蛋白质 P987 的灵敏度分别为 88%、86%、78%、74%、66% 和 60%。此外，这些蛋白质被用于确定两个不同商业畜群的血清阳性率（来自被认为没有猪肺炎支原体的农场的 254 头血清猪）和 246 个来自具有地方性肺炎临床症状和猪肺炎支原体血清学阳性的农场），P95 的阳性率为 65.2%，P102 为 54.6%，P46 为 40.2%，P97 类为 37.2%，假设的 P987 为 17.4% , Lppt 蛋白为 14%。此外，使用 118 个随机猪血清样本，将六种重组蛋白的 ELISA 与商业 HerdCheck 试剂盒进行比较，结果表明，重组蛋白的 ELISA 比商业测试更灵敏。这些数据表明，重组蛋白 P95 和 P102 是用于诊断测试以检测抗猪肺炎支原体抗体的潜在目标. 尽管还需要更多的研究，但这项研究提供的见解表明，这些重组蛋白可用于流行病学调查，并可作为区分受感染动物和接种疫苗动物的潜在生物标志物。