当前位置:
X-MOL 学术
›
Acta Biochim. Biophys. Sin.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Protective mechanisms of Agrimonia pilosa Ledeb in dextran sodium sulfate-induced colitis as determined by a network pharmacology approach
Acta Biochimica et Biophysica Sinica ( IF 3.7 ) Pub Date : 2021-09-15 , DOI: 10.1093/abbs/gmab116 Chen Li 1 , Meng Wang 1 , Junkang Sui 2 , Yongkun Zhou 1 , Wei Chen 3
中文翻译:
由网络药理学方法确定的仙鹤草在葡聚糖硫酸钠诱导的结肠炎中的保护机制
更新日期:2021-10-12
Acta Biochimica et Biophysica Sinica ( IF 3.7 ) Pub Date : 2021-09-15 , DOI: 10.1093/abbs/gmab116 Chen Li 1 , Meng Wang 1 , Junkang Sui 2 , Yongkun Zhou 1 , Wei Chen 3
Affiliation
Abstract
Previous studies reported that Agrimonia pilosa (AP) Ledeb possessed diverse biological activities, including anti-inflammatory, antioxidant, and anti-tumor activities. However, the effect of AP on ulcerative colitis (UC) remains unclear. In this study, we investigated the therapeutic effect and mechanisms of AP on dextran sodium sulfate (DSS)-induced colitis. The potential constituents of AP were investigated by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). A total of 13 compounds were recognized by UPLC-Q-TOF/MS chromatogram. Furthermore, a network pharmacology approach revealed that there are 297 candidate targets of UC and 549 common targets for the 13 active ingredients of AP. GO enrichment and KEGG pathway analysis indicated that AP might have a protective effect on UC through the nuclear factor κB (NF-κB) and nucleotide-binding oligomerization domain (NOD)-like receptor signaling pathways. Subsequent experimental validation in a DSS-induced colitis model revealed that AP alleviated the severity of DSS-induced colitis, reduced the production of proinflammatory factors, and protected against the loss of intestinal integrity. Moreover, AP inhibited the phosphorylation of NF-κB p65 and the activation of the NLRP3 inflammasome. In conclusion, AP ameliorated DSS-induced colitis through suppressing the activation of the NLRP3 inflammasome and NF-κB signaling pathways.
中文翻译:
由网络药理学方法确定的仙鹤草在葡聚糖硫酸钠诱导的结肠炎中的保护机制
摘要
以前的研究报告说,仙鹤草(AP) Ledeb 具有多种生物活性,包括抗炎、抗氧化和抗肿瘤活性。然而,AP 对溃疡性结肠炎 (UC) 的影响仍不清楚。在这项研究中,我们研究了AP对葡聚糖硫酸钠(DSS)诱导的结肠炎的治疗效果和机制。通过超高效液相色谱结合四极杆飞行时间质谱 (UPLC-Q-TOF/MS) 研究 AP 的潜在成分。UPLC-Q-TOF/MS 色谱图共识别出 13 种化合物。此外,网络药理学方法揭示了 UC 的 297 个候选靶点和 AP 的 13 种活性成分的 549 个共同靶点。GO富集和KEGG通路分析表明,AP可能通过核因子κB(NF-κB)和核苷酸结合寡聚结构域(NOD)样受体信号通路对UC产生保护作用。随后在 DSS 诱导的结肠炎模型中进行的实验验证表明,AP 可减轻 DSS 诱导的结肠炎的严重程度,减少促炎因子的产生,并防止肠道完整性的丧失。此外,AP 抑制 NF-κB p65 的磷酸化和 NLRP3 炎性体的激活。总之,AP 通过抑制 NLRP3 炎性体和 NF-κB 信号通路的激活来改善 DSS 诱导的结肠炎。随后在 DSS 诱导的结肠炎模型中进行的实验验证表明,AP 可减轻 DSS 诱导的结肠炎的严重程度,减少促炎因子的产生,并防止肠道完整性的丧失。此外,AP 抑制 NF-κB p65 的磷酸化和 NLRP3 炎性体的激活。总之,AP 通过抑制 NLRP3 炎性体和 NF-κB 信号通路的激活来改善 DSS 诱导的结肠炎。随后在 DSS 诱导的结肠炎模型中进行的实验验证表明,AP 可减轻 DSS 诱导的结肠炎的严重程度,减少促炎因子的产生,并防止肠道完整性的丧失。此外,AP 抑制 NF-κB p65 的磷酸化和 NLRP3 炎性体的激活。总之,AP 通过抑制 NLRP3 炎性体和 NF-κB 信号通路的激活来改善 DSS 诱导的结肠炎。