Plants depend on light for energy production. However, the UV component in sunlight also inflicts DNA damage, mostly in the form of cyclobutane pyrimidine dimers (CPD) and (6-4) pyrimidine–pyrimidone photoproducts, which are mutagenic and lethal to the plant cells. These lesions are repaired by blue-light-dependent photolyases and the nucleotide excision repair enzymatic systems. Here, we characterize nucleotide excision repair in Arabidopsis thaliana genome-wide and at single nucleotide resolution with special focus on transcription-coupled repair and the role of the CSA1 and CSA2 genes/proteins in dictating the efficiency and the strand preference of repair of transcribed genes. We demonstrate that CSA1 is the dominant protein in coupling repair to transcription with minor contribution from CSA2.

中文翻译：

---

拟南芥中环丁烷嘧啶二聚体的全基因组切除修复图谱以及 CSA1 和 CSA2 蛋白在转录偶联修复中的作用†

植物依靠光来产生能量。然而，阳光中的紫外线成分也会造成 DNA 损伤，主要以环丁烷嘧啶二聚体 (CPD) 和 (6-4) 嘧啶-嘧啶酮光产物的形式存在，它们对植物细胞具有致突变性和致死性。这些损伤由蓝光依赖性光解酶和核苷酸切除修复酶系统修复。在这里，我们描述了拟南芥全基因组和单核苷酸分辨率的核苷酸切除修复，特别关注转录偶联修复以及 CSA1 和 CSA2 基因/蛋白质在决定转录基因修复效率和链偏好方面的作用. 我们证明 CSA1 是偶联修复与转录的主要蛋白，CSA2 的贡献较小。