Previously, we reported the detection of two novel bluetongue virus (BTV) strains (SPvvvv/02 and SPvvvv/03), possibly representing new BTV genotypes, in a batch of sheeppox vaccine. We developed type-specific RT-qPCR assays (targeting genome segment 2) for these two new BTV strains. The limit of detection of both assays was 10 genome copies/μl and no cross-reactivity with other BTV genotypes was observed. The performance of three other BTV group-specific diagnostic assays was also tested against the putative novel genotypes. RT-qPCR assays targeting BTV segment 9 and 10 detected both strains (SPvvvv/02 and SPvvvv/03) whereas a BTV segment 1 RT-qPCR assay was unable to detect either BTV strain. The work presented here expands upon the current repertoire of RT-qPCR assays for BTV genotype determination.

此前，我们报道了在一批羊痘疫苗中检测到两种新的蓝舌病毒 (BTV) 毒株（SPvvvv/02 和 SPvvvv/03），可能代表新的 BTV 基因型。我们为这两种新的 BTV 菌株开发了类型特异性 RT-qPCR 测定（靶向基因组片段 2）。两种测定的检测限均为 10 个基因组拷贝/μl，并且未观察到与其他 BTV 基因型的交叉反应。还针对推定的新基因型测试了其他三种 BTV 组特异性诊断测定的性能。针对 BTV 片段 9 和 10 的 RT-qPCR 测定检测到两种菌株（SPvvvv/02 和 SPvvvv/03），而 BTV 片段 1 RT-qPCR 测定无法检测任何 BTV 菌株。这里介绍的工作扩展了当前用于 BTV 基因型测定的 RT-qPCR 检测方法。