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Master regulator NtrC controls the utilization of alternative nitrogen sources in Pseudomonas stutzeri A1501
World Journal of Microbiology and Biotechnology ( IF 4.1 ) Pub Date : 2021-09-15 , DOI: 10.1007/s11274-021-03144-w
Zhimin Yang 1 , Qin Li 1 , Yongliang Yan 1 , Xiubin Ke 1 , Yueyue Han 1 , Shaoyu Wu 1 , Fanyang Lv 1 , Yahui Shao 1 , Shanshan Jiang 1 , Min Lin 1 , Yunhua Zhang 1, 2 , Yuhua Zhan 1
Affiliation  

Pseudomonas stutzeri A1501 is a model strain used to study associative nitrogen fixation, and it possesses the nitrogen regulatory NtrC protein in the core genome. Nitrogen sources represent one of the important factors affecting the efficiency of biological nitrogen fixation in the natural environment. However, the regulation of NtrC during nitrogen metabolism in P. stutzeri A1501 has not been clarified. In this work, a phenotypic analysis of the ntrC mutant characterized the roles of NtrC in nitrogen metabolism and the oxidative stress response of P. stutzeri A1501. To systematically identify NtrC-controlled gene expression, RNA-seq was performed to further analyse the gene expression differences between the wild-type strain and the ∆ntrC mutant under nitrogen fixation conditions. A total of 1431 genes were found to be significantly altered by ntrC deletion, among which 147 associative genes had NtrC-binding sites, and the pathways for nitrogen fixation regulation, nitrogenous compound acquisition and catabolism and nitrate assimilation were discussed. Furthermore, the oxidative stress-related gene (katB), which was upregulated by ntrC deletion, was suggested to be a potential target gene of NtrC, thus highlighting the importance of NtrC in nitrogenase protection against oxygen damage. Based on these findings, we propose that NtrC is a high-ranking element in the regulatory network of P. stutzeri A1501 that controls a variety of nitrogen metabolic and oxidative stress responsive traits required for adaptation to complex rhizosphere environments.



中文翻译:

主调节器 NtrC 控制施氏假单胞菌 A1501 中替代氮源的利用

施氏假单胞菌A1501是一种用于研究联合固氮的模型菌株,它在核心基因组中具有氮调节NtrC蛋白。氮源是影响自然环境中生物固氮效率的重要因素之一。然而,在P. stutzeri A1501 氮代谢过程中 NtrC 的调节尚未阐明。在这项工作中,ntrC突变体的表型分析表征了 NtrC 在氮代谢和P. stutzeri A1501 的氧化应激反应中的作用。为了系统地识别 NtrC 控制的基因表达,进行 RNA-seq 以进一步分析野生型菌株和 Δ 之间的基因表达差异固氮条件下的ntrC突变体。共发现1431个基因因ntrC缺失而发生显着改变,其中147个相关基因具有NtrC结合位点,并讨论了固氮调控、含氮化合物获取和分解代谢以及硝酸盐同化的途径。此外,通过ntrC缺失上调的氧化应激相关基因(katB)被认为是NtrC的潜在靶基因,从而突出了NtrC在固氮酶保护免受氧损伤中的重要性。基于这些发现,我们建议 NtrC 是P. stutzeri监管网络中的高级元素A1501 控制适应复杂根际环境所需的多种氮代谢和氧化应激反应性状。

更新日期:2021-09-15
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