ABSTRACT

Purple passion fruit, one of the most important fruit exports in Colombia, has been seriously affected in recent years by several viral diseases. The aim of the present study was to investigate the virome of asymptomatic purple passion fruit samples from commercial plots and nurseries in Antioquia (Colombia). The presence of viruses was first tested by high-throughput sequencing (HTS) of bulked leaf samples using total RNA and dsRNA inputs. Interestingly, the HTS data suggested the presence of a new badnavirus with a genome of 6,951 bp, which was confirmed by DNA sequencing of rolling circle amplification products. This virus has been tentatively named Badnavirus passiflorae as it is the first report of a badnavirus infecting passion fruit. The presence of Soybean mosaic virus (SMV), Cowpea aphid-borne mosaic virus (CABMV), Cucumber mosaic virus (CMV), and Passion fruit yellow mosaic virus (PFYMV) was further investigated using RT-qPCR, as well as evaluated the infection by begomoviruses and badnaviruses through PCR. Using these techniques, B. passiflorae was detected in all the samples, PFYMV in 20% of adult plants and 10% of plantlets, and SMV in 20% of adult plants and 30% of plantlets. None of the samples tested positive for CABMV, CMV, or begomoviruses.

摘要

紫色百香果是哥伦比亚最重要的水果出口之一，近年来受到多种病毒性疾病的严重影响。本研究的目的是调查来自安蒂奥基亚（哥伦比亚）商业地块和苗圃的无症状紫色百香果样品的病毒组。首先通过使用总 RNA 和 dsRNA 输入的散装叶片样本的高通量测序 (HTS) 测试病毒的存在。有趣的是，HTS 数据表明存在一种基因组为 6,951 bp 的新型 badnavirus，这已通过滚环扩增产物的 DNA 测序得到证实。这种病毒被暂时命名为Badnavirus passiflorae，因为它是第一个关于感染百香果的 badnavirus 的报道。大豆花叶病毒(SMV)的存在，使用 RT-qPCR 进一步研究了豇豆蚜传花叶病毒(CABMV)、黄瓜花叶病毒(CMV) 和百香果黄色花叶病毒(PFYMV)，并通过 PCR 评估了 begomoviruses 和 badnaviruses 的感染。使用这些技术，在所有样品中检测到西番莲，20% 的成年植物和 10% 的幼苗中检测到PFYMV，20% 的成年植物和 30% 的幼苗中检测到 SMV。所有样本均未检测出 CABMV、CMV 或贝戈莫病毒呈阳性。