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A simple, sensitive and quantitative FACS-based test for SARS-CoV-2 serology in humans and animals
medRxiv - Infectious Diseases Pub Date : 2022-03-25 , DOI: 10.1101/2021.09.06.21262027
Agnes Maurel Ribes , Pierre Bessiere , Jean-Charles Guery , Eloise Joly Featherstone , Timothee Bruel , Remy Robinot , Olivier Schwartz , Florence Abravanel , Jacques Izopet , Etienne Joly

Serological tests are important for understanding the physiopathology and following the evolution of the Covid-19 pandemic. Assays based on flow cytometry (FACS) of tissue culture cells expressing the spike (S) protein of SARS-CoV-2 have repeatedly proven to perform slightly better than the plate-based assays ELISA and CLIA (chemiluminescent immuno-assay), and markedly better than lateral flow immuno-assays (LFIA).

中文翻译:

一种基于 FACS 的简单、灵敏和定量的人类和动物 SARS-CoV-2 血清学检测

血清学测试对于了解病理生理学和跟踪 Covid-19 大流行的演变非常重要。基于流式细胞术 (FACS) 对表达 SARS-CoV-2 刺突 (S) 蛋白的组织培养细胞进行的检测已反复证明其性能略好于基于平板的检测 ELISA 和 CLIA(化学发光免疫检测),并且显着优于侧流免疫测定法 (LFIA)。
更新日期:2022-03-25
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