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Arabidopsis annexin 5 is involved in maintenance of pollen membrane integrity and permeability
Journal of Experimental Botany ( IF 6.9 ) Pub Date : 2021-09-14 , DOI: 10.1093/jxb/erab419
Małgorzata Lichocka 1 , Magdalena Krzymowska 1 , Magdalena Górecka 1 , Jacek Hennig 1
Affiliation  

In Arabidopsis, a dry stigma surface enables a gradual hydration of pollen grains by a controlled release of water. Occasionally the grains may be exposed to extreme precipitations that cause rapid water influx and swelling, eventually leading to pollen membrane rupture. In metazoans, calcium- and phospholipid-binding proteins, referred to as annexins, participate in the repair of plasma membrane damages. It remains unclear, however, how this process is conducted in plants. Here, we examined whether plant annexin 5 (ANN5), the most abundant member of the annexin family in pollen, is involved in the restoration of pollen membrane integrity. We analyzed the cellular dynamics of ANN5 in pollen grains undergoing hydration in favorable or stress conditions. We observed a transient association of ANN5 with the pollen membrane during in vitro hydration that did not occur in the pollen grains being hydrated on the stigma. To simulate a rainfall, we performed spraying of the pollinated stigma with deionized water that induced ANN5 accumulation at the pollen membrane. Interestingly, calcium or magnesium application affected pollen membrane properties differently, causing rupture or shrinkage of pollen membrane, respectively. Both treatments, however, induced ANN5 recruitment to the pollen membrane. Our data suggest a model in which ANN5 is involved in the maintenance of membrane integrity in pollen grains exposed to osmotic or ionic imbalances.

中文翻译:

拟南芥膜联蛋白 5 参与维持花粉膜的完整性和通透性

在拟南芥中,干燥的柱头表面可以通过控制水的释放使花粉粒逐渐水合。有时,谷物可能会暴露在极端降水中,导致水快速流入和膨胀,最终导致花粉膜破裂。在后生动物中,钙和磷脂结合蛋白(称为膜联蛋白)参与质膜损伤的修复。然而,目前尚不清楚这一过程是如何在植物中进行的。在这里,我们检查了植物膜联蛋白 5 (ANN5),花粉中膜联蛋白家族中最丰富的成员,是否参与了花粉膜完整性的恢复。我们分析了在有利或压力条件下经历水合的花粉粒中 ANN5 的细胞动力学。我们观察到 ANN5 与花粉膜在体外水合过程中的短暂关联,这在柱头上水合的花粉粒中没有发生。为了模拟降雨,我们用去离子水喷洒授粉柱头,诱导 ANN5 在花粉膜上积累。有趣的是,钙或镁的应用对花粉膜特性的影响不同,分别导致花粉膜破裂或收缩。然而,这两种处理都诱导了 ANN5 向花粉膜的募集。我们的数据表明了一个模型,其中 ANN5 参与维持暴露于渗透或离子失衡的花粉粒中的膜完整性。我们用去离子水喷洒授粉柱头,诱导 ANN5 在花粉膜上积累。有趣的是,钙或镁的应用对花粉膜特性的影响不同,分别导致花粉膜破裂或收缩。然而,这两种处理都诱导了 ANN5 向花粉膜的募集。我们的数据表明了一个模型,其中 ANN5 参与维持暴露于渗透或离子失衡的花粉粒中的膜完整性。我们用去离子水喷洒授粉柱头,诱导 ANN5 在花粉膜上积累。有趣的是,钙或镁的应用对花粉膜特性的影响不同,分别导致花粉膜破裂或收缩。然而,这两种处理都诱导了 ANN5 向花粉膜的募集。我们的数据表明了一个模型,其中 ANN5 参与维持暴露于渗透或离子失衡的花粉粒中的膜完整性。
更新日期:2021-09-14
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