Summary Recent efforts to identify novel bacterial structured noncoding RNA (ncRNA) motifs through searching long, GC-rich intergenic regions (IGRs) have revealed several new classes, including the recently validated HMP-PP riboswitch. The DIMPL (Discovery of Intergenic Motifs PipeLine) discovery pipeline described herein enables rapid extraction and selection of bacterial IGRs that are enriched for structured ncRNAs. Moreover, DIMPL automates the subsequent computational steps necessary for their functional identification. Availability and implementation The DIMPL pipeline is freely available as a Docker image with an accompanying set of Jupyter notebooks. Full instructions for download and use are available at https://github.com/breakerlab/dimpl. Supplementary information Supplementary data are available at Bioinformatics online.

中文翻译：

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DIMPL：用于发现细菌中结构化非编码 RNA 基序的生物信息学管道

总结 最近通过搜索富含 GC 的长基因间区域 (IGR) 来识别新型细菌结构化非编码 RNA (ncRNA) 基序的努力揭示了几个新类别，包括最近验证的 HMP-PP 核糖开关。本文描述的 DIMPL（基因间基序管道的发现）发现管道能够快速提取和选择富含结构化 ncRNA 的细菌 IGR。此外，DIMPL 自动执行其功能识别所需的后续计算步骤。可用性和实施​​ DIMPL 管道可作为 Docker 映像免费提供，并附带一组 Jupyter 笔记本。有关下载和使用的完整说明，请访问 https://github.com/breakerlab/dimpl。补充信息 补充数据可在 Bioinformatics 在线获取。