Laser speckle rheology is a powerful method to measure viscoelasticity of biological fluids. To date, the in vivo assessment of viscoelastic properties of biofluids has been limited by several reasons such as combined speckle fluctuation and insufficient detection depth. In order to avoid these shortcomings, in particular, the problems associated with detection depth during in vivo viscoelastic testing of biological fluids, the transmission method has been adapted in this work. According to the experimental results, the modified laser speckle rheology technique has enabled one to increase a speckle fluctuation range by nine times at keeping the same thickness of the biomimetic materials, which is of great significance for further in-vivo research.

激光散斑流变学是测量生物体液粘弹性的有效方法。迄今为止，生物流体粘弹性的体内评估受到多种原因的限制，例如组合斑点波动和检测深度不足。为了避免这些缺点，特别是在生物流体的体内粘弹性测试过程中与检测深度相关的问题，在这项工作中采用了传输方法。实验结果表明，改进后的激光散斑流变技术在保持仿生材料厚度不变的情况下，使散斑波动范围增加了9倍，这对进一步的体内研究具有重要意义。