Baesd on previous researches, the natural deep eutectic solvent (DES) has enormous potential to be used in the fabrication of hydrogel wound dressing due to its outstanding properties including cytocompatibility, degradability and solubility. In order to further improve the antibaterial capacity of hydrogel, in the present study sodium hyaluronate (SH) and the natural DES were utilized to develop a novel hydrogel wound dressing by dopamine (DA) coated SH with in situ reduction of silver nanoparticles (DES-DASH@Ag). Furthermore, during the preparation process, we discovered for the first time that the DES can be used to fill the freeze-dried DASH to prepare a hydrogel (DES-DASH), which was promising to utilized in the fabrication of other hydrogels. Besides, the chemical and physical properties as well as wound healing capacity of the DES-DASH@Ag hydrogel were characterized. As a result, the DES-DASH@Ag hydrogel presented good cytocompatibility tested using NIH-3 T3 fibroblast cells, and prominent antibacterial effect against two types of bacteria infecting exposed wound. Furthermore, the hydrogel facilitated regeneration of mouse skin tissue in the wound area. The overall performance of DES-DASH@Ag hydrogel suggested that it could be a promising wound dressing in modern medicine.

基于以往的研究，天然深共熔溶剂（DES）由于其优异的细胞相容性、降解性和溶解性等特性，在制备水凝胶伤口敷料方面具有巨大的潜力。为了进一步提高水凝胶的抗菌能力，在本研究中，利用透明质酸钠 (SH) 和天然 DES 开发了一种由多巴胺 (DA)原位涂覆的新型水凝胶伤口敷料。银纳米颗粒（DES-DASH@Ag）的还原。此外，在制备过程中，我们首次发现DES可用于填充冷冻干燥的DASH制备水凝胶（DES-DASH），有望用于其他水凝胶的制备。此外，还表征了 DES-DASH@Ag 水凝胶的化学和物理特性以及伤口愈合能力。因此，DES-DASH@Ag 水凝胶在使用 NIH-3 T3 成纤维细胞进行测试时表现出良好的细胞相容性，并对感染暴露伤口的两种细菌具有显着的抗菌作用。此外，水凝胶促进了伤口区域小鼠皮肤组织的再生。DES-DASH@Ag 水凝胶的整体性能表明它可能是现代医学中一种很有前途的伤口敷料。