In this study, the fibrinogen imprinted surface plasmon resonance (SPR) biosensor was developed for the monitoring of fibrinogen levels in patient serum samples using a microcontact imprinting method. The fibrinogen imprinted and non-imprinted polymeric films on the SPR chip surface were prepared by the microcontact imprinting approach and characterized by ellipsometer, contact angle measurements, and Fourier transform infrared spectroscopy. The limit of detection and quantification values of the fibrinogen imprinted SPR biosensors in the linear range of 0.05–9.0 mg/mL were 0.00066 and 0.00219 mg/mL, respectively. In all kinetic analyzes, the response time was 11 min for equilibration, adsorption, and desorption cycles. The selectivity studies of SPR biosensors were performed in the presence of fibrinogen, myoglobin, hemoglobin, and immunoglobulin G in a competitive manner. For the validation studies of the fibrinogen imprinted SPR biosensor, the fibrinogen level of diluted patient serum samples was determined by enzyme-linked immunosorbent assay (ELISA). The experimental results show that the serum samples of patients in high and low-risk groups were identified with the recovery of about % 97–99 according to both SPR biosensor and ELISA analysis results.

中文翻译：

---

通过印迹识别位点实时检测纤维蛋白原

在这项研究中，开发了纤维蛋白原印迹表面等离子体共振 (SPR) 生物传感器，用于使用微接触印迹方法监测患者血清样品中的纤维蛋白原水平。SPR 芯片表面的纤维蛋白原印迹和非印迹聚合物膜是通过微接触印迹方法制备的，并通过椭偏仪、接触角测量和傅里叶变换红外光谱进行表征。纤维蛋白原印迹 SPR 生物传感器在 0.05–9.0 mg/mL 线性范围内的检测限和定量值分别为 0.00066 和 0.00219 mg/mL。在所有动力学分析中，平衡、吸附和解吸循环的响应时间为 11 分钟。SPR 生物传感器的选择性研究是在纤维蛋白原、肌红蛋白、血红蛋白、和免疫球蛋白 G 以竞争方式。对于纤维蛋白原印迹 SPR 生物传感器的验证研究，通过酶联免疫吸附测定 (ELISA) 确定稀释的患者血清样品的纤维蛋白原水平。实验结果表明，根据SPR生物传感器和ELISA分析结果，对高危组和低危组患者的血清样本进行鉴定，回收率约为97-99%。