Evolving testing methods in food Microbiology have resulted in the need for different types of microbiological reference materials. Based on the research articles available in this area, it is evident that development has been quite substantial in chemical testing compared to Microbiology. The primary reason could be the ease of spiking, and recovery in chemical RM as compared to microbiological RM. A significant challenge faced in recovery and stability during the development of quantitative microbiological RM depends on temperature, type of microbiological media used, method of analysis including reconstitution method, interference due to antimicrobial agents in food matrices, and competitor microorganisms present in higher numbers then the target microorganisms. Most of the research papers published on microbiological reference materials are contributed by developed economies were in the information related to complex food matrices are limited. Further analysis of different International Depository Agencies under the Budapest treaty indicates that there are only three institutes based in Europe providing quantitative or assigned value RM. This, in turn, highlights the scarcity in the availability of quantitative RM in Microbiology. This article provides a global overview of the availability of microbiological RM, microbial preservation techniques, protective agents, and elaboration on developing different formats of microbiological RM used in food testing.

食品微生物学中不断发展的测试方法导致需要不同类型的微生物参考材料。根据该领域现有的研究文章，很明显，与微生物学相比，化学测试的发展相当可观。主要原因可能是与微生物 RM 相比，化学 RM 易于加标和回收。在定量微生物 RM 的开发过程中，在回收率和稳定性方面面临的一个重大挑战取决于温度、所用微生物介质的类型、分析方法（包括重构方法）、食品基质中抗微生物剂的干扰以及存在的竞争微生物数量高于目标微生物。大多数发表在微生物参考材料上的研究论文是由发达经济体贡献的，与复杂食品基质相关的信息有限。对布达佩斯条约下不同国际存管机构的进一步分析表明，欧洲只有三个机构提供定量或指定价值的 RM。这反过来又凸显了微生物学中定量 RM 的可用性的稀缺性。本文概述了微生物 RM 的可用性、微生物保存技术、保护剂，并详细阐述了开发用于食品检测的不同形式的微生物 RM。对布达佩斯条约下不同国际存管机构的进一步分析表明，欧洲只有三个机构提供定量或指定价值的 RM。这反过来又凸显了微生物学中定量 RM 的可用性的稀缺性。本文概述了微生物 RM 的可用性、微生物保存技术、保护剂，并详细阐述了开发用于食品检测的不同形式的微生物 RM。对布达佩斯条约下不同国际存管机构的进一步分析表明，欧洲只有三个机构提供定量或指定价值的 RM。这反过来又凸显了微生物学中定量 RM 的可用性的稀缺性。本文概述了微生物 RM 的可用性、微生物保存技术、保护剂，并详细阐述了开发用于食品检测的不同形式的微生物 RM。