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Streptomyces sp. JCK-6131 Protects Plants Against Bacterial and Fungal Diseases via Two Mechanisms
Frontiers in Plant Science ( IF 5.6 ) Pub Date : 2021-09-15 , DOI: 10.3389/fpls.2021.726266
Khanh Duy Le 1 , Jeun Kim 2 , Hoa Thi Nguyen 1 , Nan Hee Yu 1 , Ae Ran Park 1 , Chul Won Lee 2 , Jin-Cheol Kim 1
Affiliation  

Plant bacterial and fungal diseases cause significant agricultural losses and need to be controlled. Beneficial bacteria are promising candidates for controlling these diseases. In this study, Streptomyces sp. JCK-6131 exhibited broad-spectrum antagonistic activity against various phytopathogenic bacteria and fungi. In vitro assays showed that the fermentation filtrate of JCK-6131 inhibited the growth of bacteria and fungi with minimum concentration inhibitory (MIC) values of 0.31–10% and 0.31–1.25%, respectively. In the in vivo experiments, treatment with JCK-6131 effectively suppressed the development of apple fire blight, tomato bacterial wilt, and cucumber Fusarium wilt in a dose-dependent manner. RP-HPLC and ESI-MS/MS analyses indicated that JCK-6131 can produce several antimicrobial compounds, three of which were identified as streptothricin E acid, streptothricin D, and 12-carbamoyl streptothricin D. In addition, the disease control efficacy of the foliar application of JCK-6131 against tomato bacterial wilt was similar to that of the soil drench application, indicating that JCK-6131 could enhance defense resistance in plants. Molecular studies on tomato plants showed that JCK-6131 treatment induced the expression of the pathogenesis-related (PR) genes PR1, PR3, PR5, and PR12, suggesting the simultaneous activation of the salicylate (SA) and jasmonate (JA) signaling pathways. The transcription levels of PR genes increased earlier and were higher in treated plants than in untreated plants following Ralstonia solanacearum infection. These results indicate that Streptomyces sp. JCK-6131 can effectively control various plant bacterial and fungal diseases via two distinct mechanisms of antibiosis and induced resistance.



中文翻译:

链霉菌属 JCK-6131 通过两种机制保护植物免受细菌和真菌病害

植物细菌和真菌病害造成重大农业损失,需要加以控制。有益细菌是控制这些疾病的有希望的候选者。在这项研究中,链霉菌sp. JCK-6131 对多种植物病原细菌和真菌表现出广谱拮抗活性。体外化验表明 JCK-6131 的发酵滤液抑制细菌和真菌的生长,最小浓度抑制 (MIC) 值分别为 0.31-10% 和 0.31-1.25%。在里面体内 实验表明,JCK-6131 处理有效抑制了苹果火疫病、番茄青枯病和黄瓜的发展 镰刀菌以剂量依赖性方式枯萎。RP-HPLC 和 ESI-MS/MS 分析表明 JCK-6131 可以产生多种抗菌化合物,其中三种被鉴定为链丝菌素 E 酸、链丝菌素 D 和 12-氨基甲酰基链丝菌素 D。 JCK-6131 叶面喷施对番茄青枯病的防治效果与土壤淋施相似,表明 JCK-6131 可增强植物的防御抗性。对番茄植物的分子研究表明,JCK-6131 处理诱导了病机相关 (公关) 基因 PR1, PR3, PR5, 和 PR12,表明同时激活水杨酸 (SA) 和茉莉酸 (JA) 信号通路。转录水平公关 基因在经过处理的植物中比未处理的植物中增加的更早并且更高 青枯病菌感染。这些结果表明链霉菌sp. JCK-6131 可以通过两种不同的抗菌和诱导抗性机制有效控制各种植物细菌和真菌病害。

更新日期:2021-09-15
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