Previous studies showed altered angiopoietin-like protein-8 (ANGPTL-8) and resistin circulating levels in type 2 diabetes mellitus (T2DM). Whether or not the alteration in ANGPTL-8 and resistin level can be a predictive maker for increased diabetic nephropathy risk remains unclear.

To Investigate the possible association of ANGPTL-8 and resistin with DN, and whether this association is affected by NAFLD status.

A total of 278 T2DM patients were enrolled. Serum levels of ANGPTL8, resistin, BMI, blood pressure, duration of diabetes, glycosylated hemoglobin (HbA1c), fasting blood glucose (FPG), hypersensitive C-reactive protein (hs-CRP), lipid profile, liver, and kidney function tests were assessed. The relationship between DN with ANGPTL8 and resistin was analyzed in the unadjusted and multiple-adjusted regression models.

Serum levels of ANGPTL8 and resistin were significantly higher in DN compared with T2DM subjects without DN (respectively; P <0.001), especially in non-NAFLD populations. ANGPTL8 and resistin showed positive correlation with hs-CRP (respectively; P<0.01), and negative correlation with estimated GFR (eGFR) (respectively; P=<0.001) but no significant correlation to HOMA-IR(respectively; P>0.05). Analysis showed ANGPTL8 levels were positively associated with resistin but only in T2DM patients with DN(r=0.1867; P<0.05), and this significant correlation disappeared in T2DM patients without DN. After adjusting for confounding factors, both ANGPTL8(OR=2.095, 95%CI 1.253-3.502 P=0.005) and resistin (OR=2.499, 95%CI 1.484-4.208 P=0.001) were risk factors for DN. Data in non-NAFLD population increased the relationship between ANGPTL8 (OR=2.713, 95% CI 1.494-4.926 P=0.001), resistin (OR=4.248, 95% CI 2.260-7.987 P<0.001)and DN. The area under the curve (AUC) on receiver operating characteristic (ROC) analysis of the combination of ANGPTL8 and resistin was 0.703, and the specificity was 70.4%. These data were also increased in non-NAFLD population, as the AUC (95%CI) was 0.756, and the specificity was 91.2%.

This study highlights a close association between ANGPTL8, resistin and DN, especially in non-NAFLD populations. These results suggest that ANGPTL-8 and resistin may be risk predictors of DN.

先前的研究表明，在 2 型糖尿病 (T2DM) 中，血管生成素样蛋白 8 (ANGPTL-8) 和抵抗素循环水平发生了改变。ANGPTL-8 和抵抗素水平的改变是否可以成为糖尿病肾病风险增加的预测因素仍不清楚。

调查 ANGPTL-8 和抵抗素与 DN 的可能关联，以及这种关联是否受 NAFLD 状态的影响。

共招募了 278 名 T2DM 患者。ANGPTL8、抵抗素、BMI、血压、糖尿病病程、糖化血红蛋白（HbA1c）、空腹血糖（FPG）、过敏性C反应蛋白（hs-CRP）、血脂、肝肾功能的血清水平是评估。在未调整和多重调整的回归模型中分析了DN与ANGPTL8和抵抗素之间的关系。

与没有 DN 的 T2DM 受试者相比，DN 中 ANGPTL8 和抵抗素的血清水平显着更高（分别；P <0.001），尤其是在非 NAFLD 人群中。ANGPTL8 和抵抗素与 hs-CRP 呈正相关（分别；磷<0.01)，并且与估计的 GFR (eGFR) 负相关（分别；磷=<0.001) 但与 HOMA-IR 无显着相关性(分别; P>0.05)。分析显示 ANGPTL8 水平与抵抗素呈正相关，但仅在 DN 的 T2DM 患者中（r=0.1867；磷<0.05)，这种显着相关性在没有 DN 的 T2DM 患者中消失。调整混杂因素后，ANGPTL8（OR=2.095, 95%CI 1.253-3.502 P=0.005）和抵抗素（OR=2.499, 95%CI 1.484-4.208 P=0.001）均为DN的危险因素。非 NAFLD 人群的数据增加了 ANGPTL8 (OR=2.713, 95% CI 1.494-4.926 P=0.001)、抵抗素 (OR=4.248, 95% CI 2.260-7.987 P<0.001) 和 DN 之间的关系。ANGPTL8和抵抗素组合的受试者工作特征(ROC)分析曲线下面积(AUC)为0.703，特异性为70.4%。这些数据在非 NAFLD 人群中也有所增加，因为 AUC (95% CI) 为 0.756，特异性为 91.2%。

该研究强调了 ANGPTL8、抵抗素和 DN 之间的密切关联，尤其是在非 NAFLD 人群中。这些结果表明 ANGPTL-8 和抵抗素可能是 DN 的风险预测因子。