Research question

Is there any difference in live birth rate between the natural cycle and hormone replacement therapy (HRT) endometrial preparation protocols for women with regular menstrual cycles undergoing their first single vitrified–warmed euploid blastocyst transfer?

Design

This was a retrospective cohort study that enrolled 722 women who underwent vitrified–warmed euploid blastocyst transfer at assisted reproductive technology (ART) centre of The First Affiliated Hospital of Zhengzhou University, from January 2013 to December 2019. Univariate and multivariate logistic regression models were used to analyse the relationship between the endometrial preparation protocols and live birth rates. Stratified analyses and sensitivity analyses were performed to ensure the reliability and stability of the results.

Results

A total of 722 single vitrified–warmed euploid blastocyst transfer cycles were included. Overall, the live birth rates were 50.00% (110/220) in the natural cycle group and 47.61% (239/502) in the HRT group. Multiple logistic regression analyses showed that there was no significant association (adjusted odds ratio 0.82; 95% confidence interval 0.56–1.20; P = 0.313) between natural cycle and HRT protocols and the live birth rate. Interaction analysis showed that there was no significant difference in live birth rates between the two groups for any subgroup after adjusting for confounding factors.

Conclusions

For single vitrified–warmed euploid blastocyst transfer, natural cycle and HRT endometrial preparation protocols result in similar live birth rates among women with regular menstrual cycles. Further studies are needed into the effects of endometrial preparation protocols on pregnancy outcomes.

中文翻译：

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单整倍体囊胚移植的自然周期与激素替代疗法后的活产率：一项回顾性队列研究

研究问题

自然周期和激素替代疗法 (HRT) 子宫内膜制备方案对于有规律月经周期的女性进行首次单一玻璃化加热整倍体囊胚移植的活产率是否存在差异？

设计

这是一项回顾性队列研究，纳入了 2013 年 1 月至 2019 年 12 月在郑州大学第一附属医院辅助生殖技术（ART）中心接受玻璃化加热整倍体囊胚移植的 722 名女性。使用单变量和多变量逻辑回归模型分析子宫内膜准备方案与活产率之间的关系。进行分层分析和敏感性分析，以确保结果的可靠性和稳定性。

结果

总共包括 722 个单一玻璃化加热的整倍体囊胚移植周期。总体而言，自然周期组的活产率为 50.00% (110/220)，HRT 组为 47.61% (239/502)。 多元逻辑回归分析表明，自然周期和 HRT 方案与活产率之间没有显着关联（调整优势比 0.82；95% 置信区间 0.56-1.20；P = 0.313）。交互作用分析显示，调整混杂因素后，任何亚组的两组活产率均无显着差异。

结论

对于单一玻璃化加热的整倍体囊胚移植，自然周期和 HRT 子宫内膜制备方案导致月经周期正常的女性的活产率相似。需要进一步研究子宫内膜准备方案对妊娠结局的影响。