As an essential gasotransmitter, carbon monoxide (CO) had gradually become a research hotspot in that it possessed important physiological functions and unique pharmacological properties. However, to date, no report has focused on the topic of detecting CO both in vivo and using films. To open up a new field of CO probes, for the first time, we designed a probe (PMAH-CO) that showed a distinctive ratio emission characteristic and displayed the quantitative distribution of CO in HeLa cells and zebrafish with a higher signal-to-noise ratio. Meanwhile, the fluorescent polysiloxane-based film (PMF) containing PMAH-CO exhibited an excellent response to CO. Due to the addition of the Si–O bond, the probe exhibited a broad transparency in the visible light range and had excellent photostability. Moreover, the probe was economically viable, easy to handle, and suitable for biological research. Hence, PMAH-CO and PMF would open up the road to broaden the application of silicone materials in the field of fluorescence imaging.

中文翻译：

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功能性聚硅氧烷使生物系统和薄膜中一氧化碳的存在可视化

一氧化碳（CO）作为一种重要的气体传递体，具有重要的生理功能和独特的药理特性，逐渐成为研究热点。然而，迄今为止，还没有报告关注在体内和使用薄膜检测 CO 的主题。为了开辟 CO 探针的新领域，我们首次设计了一种探针（PMAH-CO），该探针显示出独特的比率发射特征，并以更高的信号比显示了 HeLa 细胞和斑马鱼中 CO 的定量分布。噪声比。同时，含有PMAH-CO的荧光聚硅氧烷基薄膜 ( PMF )对 CO 表现出极好的响应。由于添加了 Si-O 键，探针在可见光范围内表现出广泛的透明度，并具有优异的光稳定性。此外，该探针在经济上可行，易于操作，适用于生物学研究。因此，PMAH-CO和PMF将为拓宽有机硅材料在荧光成像领域的应用开辟道路。