Biomolecule-functionalized Au nanoclusters (AuNCs) have drawn great interest in the electrochemiluminescence (ECL) field due to their unique optical/electrical properties, biocompatibility, and versatile bioapplication potentials. Herein, we proposed a two-in-one ECL probe of immunoglobulin G-encapsulated AuNCs (IgG-AuNCs) for the development of a high-performance ECL immunoassay (ECLIA) platform. The IgG-AuNCs were not only used as an ECL probe due to their excellent anodic ECL performance with triethylamine (TEA) as a coreactant but also used as the biorecognition element because of their well-retained bioactivity of the IgG. As a proof of concept, a new type of competitive immunosensing platform has been applied to detect IgG representing several merits of facile preparation, rapid detection, sample saving, and good analytical performance. The sensing platform exhibited a linear range of 0.5–50,000 ng/mL with a limit of detection of 0.06 ng/mL for IgG detection with high selectivity. In addition, this convenient ECLIA platform also performed well in real serum sample detection. Notably, our work not only proved the “two-in-one” immuno-AuNC probe-based ECLIA strategy but also developed a rational framework for study of ECL bioassay platforms based on multifunctional AuNCs and other related nanomaterials.

中文翻译：

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使用免疫球蛋白 G 封装的金纳米团簇作为“二合一”探针的电化学发光免疫分析平台

生物分子功能化的 Au 纳米团簇 (AuNCs) 由于其独特的光学/电学特性、生物相容性和多功能的生物应用潜力而在电化学发光 (ECL) 领域引起了极大的兴趣。在此，我们提出了一种免疫球蛋白 G 封装的 AuNCs (IgG-AuNCs) 的二合一 ECL 探针，用于开发高性能 ECL 免疫测定 (ECLIA) 平台。IgG-AuNCs 不仅由于其以三乙胺 (TEA) 作为共反应物的优异阳极 ECL 性能而被用作 ECL 探针，而且由于其良好保留的 IgG 生物活性也被用作生物识别元件。作为概念证明，一种新型的竞争性免疫传感平台已被应用于检测 IgG，其具有制备简便、检测快速、节省样品和良好的分析性能等几个优点。传感平台的线性范围为 0.5–50,000 ng/mL，检测限为 0.06 ng/mL，用于高选择性 IgG 检测。此外，这种便捷的 ECLIA 平台在实际血清样本检测中也表现良好。值得注意的是，我们的工作不仅证明了基于“二合一”免疫 AuNC 探针的 ECLIA 策略，而且还为基于多功能 AuNC 和其他相关纳米材料的 ECL 生物测定平台的研究开发了合理的框架。