Abstract

This study aims to evaluate the anti-Leishmania major and the lung adenocarcinoma (A549) cytotoxicity of Withania somnifera root and fruit. The total extracts were obtained by homogenisation in aqueous MeOH, and the sub-extracts [n-hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH), and methanol (MeOH)] were obtained by flash chromatography. The activity evaluation showed that n-BuOH sub-extracts from root and fruit exhibited noticeable antileishmanial promastigote properties. The n-hexane and EtOAc sub-extracts from both organs, and the MeOH sub-extract from the fruit exerted mild to moderate effects on the promastigotes. In-vitro growth-inhibitory test results on axenic amastigote and cytotoxicity testing on macrophages (RAW264.7), the parasite-host at the amastigote stage, revealed that the activity was mainly concentrated in the root EtOAc and n-BuOH sub-extracts and to a lesser extent the fruit MeOH and EtOAc, and the root n-hexane sub-extracts. Only the roots' EtOAc and n-BuOH sub-extracts demonstrated low cytotoxicity on the A549 cell line.

摘要

本研究旨在评估睡茄根和果实的抗大利什曼原虫和肺腺癌 (A549) 细胞毒性。通过在MeOH水溶液中均质化获得总提取物，通过快速色谱获得子提取物[正己烷、乙酸乙酯(EtOAc)、正丁醇( n- BuOH)和甲醇(MeOH)]。活性评估表明，来自根和果实的n -BuOH 子提取物表现出明显的抗螨虫前鞭毛体特性。来自两个器官的正己烷和 EtOAc 子提取物，以及来自水果的 MeOH 子提取物对前鞭毛体产生轻度至中度的影响。体外无菌无鞭毛体的生长抑制试验结果和无鞭毛体期的寄生虫宿主巨噬细胞 (RAW264.7) 的细胞毒性试验结果表明，该活性主要集中在根 EtOAc 和n -BuOH 子提取物中，而在较小的提取果实中的甲醇和乙酸乙酯，以及根正己烷子提取物。只有根的 EtOAc 和n -BuOH 子提取物对 A549 细胞系表现出低细胞毒性。