ABSTRACT

Chronic wounds have affected more than 6 million people, especially diabetic populations. Various antimicrobial substances have been added to nanocellulose-based wound dressing to inhibit microbial growth. However, the substances triggered multiple adverse implications on humans. In this communication, we have developed a ferulic acid-grafted cellulose nanocrystal film as a potential antimicrobial material for wound dressing development. Needle-like cellulose nanocrystals, with an average length of 159 ± 31 nm, were extracted from medical-grade cotton using the acid hydrolysis method. A combination of cellulose nanocrystals, polyvinyl alcohol (PVA) and ferulic acid has generated a soft, sturdy, flexible, elastic and non-ductile film. It absorbed a large amount of exudates by presenting a swelling ratio of 774 ± 57%. The release of ferulic acid achieved a plateau at 20 h, with a total release of 75.4 ± 7.9% from the developed film. The ferulic acid-grafted cellulose nanocrystal film inhibited the growth of 2 Gram-positive bacteria, 3 Gram-negative bacteria and 1 yeast. During the Hohenstein challenge and wash durability, the developed film retained its antimicrobial efficiency by reducing up to 82% microbial growth even after 20 washes. The findings exposed that the ferulic acid-grafted cellulose nanocrystal film is effective in combating wound pathogens and ideal for dressing development.

摘要

慢性伤口已经影响了超过 600 万人，尤其是糖尿病人群。各种抗菌物质已被添加到基于纳米纤维素的伤口敷料中以抑制微生物生长。然而，这些物质对人类引发了多重不利影响。在本次交流中，我们开发了一种阿魏酸接枝纤维素纳米晶体薄膜，作为用于伤口敷料开发的潜在抗菌材料。使用酸水解法从医用棉中提取出针状纤维素纳米晶体，平均长度为 159 ± 31 nm。纤维素纳米晶体、聚乙烯醇 (PVA) 和阿魏酸的组合产生了一种柔软、坚固、柔韧、有弹性和非延展性的薄膜。它通过呈现 774 ± 57% 的溶胀率吸收大量渗出液。阿魏酸的释放在 20 小时时达到稳定状态，从显影膜中的总释放量为 75.4 ± 7.9%。阿魏酸接枝的纤维素纳米晶膜抑制了 2 种革兰氏阳性菌、3 种革兰氏阴性菌和 1 种酵母菌的生长。在海恩斯坦挑战和洗涤耐久性期间，即使经过 20 次洗涤，开发的薄膜仍能减少高达 82% 的微生物生长，从而保持其抗菌效率。研究结果表明，阿魏酸接枝的纤维素纳米晶体膜可有效对抗伤口病原体，是敷料开发的理想选择。在海恩斯坦挑战和洗涤耐久性期间，即使经过 20 次洗涤，开发的薄膜仍能减少高达 82% 的微生物生长，从而保持其抗菌效率。研究结果表明，阿魏酸接枝的纤维素纳米晶体膜可有效对抗伤口病原体，是敷料开发的理想选择。在海恩斯坦挑战和洗涤耐久性期间，即使经过 20 次洗涤，开发的薄膜仍能减少高达 82% 的微生物生长，从而保持其抗菌效率。研究结果表明，阿魏酸接枝的纤维素纳米晶体膜可有效对抗伤口病原体，是敷料开发的理想选择。